SAB4200500
抗-胶原IV抗体，小鼠单克隆
clone J3-2, purified from hybridoma cell culture
别名:
单克隆抗-胶原蛋白IV 小鼠抗, 抗-COL4
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
J3-2, monoclonal
Application:
IHC, WB
Citations:
20
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
J3-2, monoclonal
form
buffered aqueous solution
species reactivity
mouse, rat, dog, bovine, human, monkey
concentration
~1.0 mg/mL
technique(s)
immunohistochemistry: 2.0-4.0 μg/mL using human liver tissue sections., western blot: 1.0-2.0 μg/mL using human placenta extracts.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... COL4A1(1282)
General description
人胶原IV包含六个基因。COL4A1-COL4A2定位于13号染色体。COL4A3-COL4A4和COL4A5-COL4A6分别位于2号染色体和X号染色体上。它属于胶原超家族，并与基底膜（BMs）相关。六个α链，即α1（IV）至α6（IV）包含N端半胱氨酸和赖氨酸结构域，典型的Gly-X-Y三重重复序列和C端非胶原（NC1）结构域。
Immunogen
胎盘制剂富含基底膜胶原蛋白。
Application
小鼠抗IV型胶原单克隆抗体可用于：
- 免疫组织化学染色
- 免疫染色
- 免疫荧光检测
- 作为免疫印迹分析的二抗
Biochem/physiol Actions
胶原蛋白IV在基底膜组装中起关键作用。六个链组装成三个不同的原体，即α 1.α1.α2（IV）、α3.α4.α5（IV）和α 5.α5.α6（IV）。三聚体以α-链的不同组合形成。在专门的肾小球BM（GBM）中观察到α3、α4和α5的高表达水平。COlA5基因的突变与Alport综合征有关，而α3（IV）链与Goodpasture综合征（一种自身免疫性疾病）的发病机制有关。COlA5中的突变也与弥漫性食管平滑肌瘤病有关。
Physical form
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
此项目有
Willi Halfter et al.
PloS one, 12(12), e0189857-e0189857 (2017-12-29)
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Sex-dependent co-occurrence of hypoxia and beta-amyloid plaques in hippocampus and entorhinal cortex is reversed by long-term treatment with ubiquinol and ascorbic acid in the 3times Tg-AD mouse model of Alzheimer's disease
Fronti?an-Rubio J, et al.
Molecular and Cellular Neurosciences, 92(1), 67-81 (2018)
Type IV collagen: structure, gene organization, and role in human diseases. Molecular basis of Goodpasture and Alport syndromes and diffuse leiomyomatosis.
Hudson BG, et al.
The Journal of Biological Chemistry, 268(35), 26033-26036 (1993)
Lysyl hydroxylase inhibition by minoxidil blocks collagen deposition and prevents pulmonary fibrosis via TGF-beta1/Smad3 signaling pathway
Shao S, et al.
Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 24(2), 8592-8592 (2018)
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