Apoptosis signal-regulating kinase 1 (ASK1) is a serine/threonine protein kinase. It belongs to the MAPKKK (mitogen-activated protein kinase) family. This gene is located on human chromosome 6q23.

Mitogen-activated protein kinase kinase kinase 5 (MAP3K5), also known as apoptosis signal-regulating kinase 1 (ASK1), is encoded by the gene mapped to human chromosome 6q22.33. The encoded protein belongs to the mitogen-activated protein kinase family. Porcine MAP3K5, which shares 97 % sequence identity with human MAP3K5, is characterized with a domain of unknown function (DUF4071) and a protein kinase domain.

The antiserum was produced against synthesized peptide derived from human ASK1 around the phosphorylation site of Ser966.

Immunogen Range: 932-981

Anti-phospho-ASK1 (pSer966) antibody has been used in western blotting.

Anti-phospho-ASK1 (pSer966) antibody produced in rabbit has been used in immunoblot analysis.

Apoptosis signal-regulating kinase 1 (ASK1) is linked with apoptosis in cancer and heart tissues. It act as an upstream regulator of p38 and JNK (c-Jun N-terminal kinase) in cardiomyocytes death. Mutations in MAP3K5 gene result in β-thalassemia disease severity and hydroxyurea treatment efficacy.

Mitogen-activated protein kinase kinase kinase 5 (MAP3K5), is one of the vital genes involved in the mitogen-activated protein kinase (MAPK) signaling cascade, which plays a crucial role in the cytoplasmic transduction of extracellular signals. Apoptosis signal-regulating kinase 1 (ASK1)-dependent mitochondrial apoptosis pathway, activated by 4SC-202, which is a novel class I HDAC inhibitor (HDACi), potentially stops hepatocellular carcinoma (HCC) cell proliferation. The encoded protein in response to oxidative stress, endoplasmic reticulum stress, infection and calcium influx plays a vital role in intracellular signaling pathways and biological functions including cell death, differentiation and inflammation. Genomic variation in MAP3K5 indicates β-thalassemia disease severity and hydroxyurea (HU) treatment efficiency. ASK1 plays a vital role in inhibition of the adipocytic differentiation process of the tumor cells and functions as an efficient therapeutic target for several human diseases including malignant fibrous histiocytomas.

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Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

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