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Merck
CN

STS0033

Agarose tablets

Low EEO, For molecular biology (200 x 500mg tablets)

别名:

3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose LE, Agarose tablets

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化学文摘社编号:
9012-36-6
UNSPSC Code:
41105317
NACRES:
NB.21
MDL number:
MFCD00081294

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SMILES string

O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

biological source

algae (marine)

grade

Molecular Biology

form

tablet

impurities

≤10.0% moisture content

color

white

EEO

0.09-0.13 (-Mr)

transition temp

transition temp 34.5-37.5 °C

gel strength

≥1200 g/cm2 (1% gel)

anion traces

sulfate (SO42-): ≤0.15%

suitability

suitable for electrophoresis, suitable for molecular biology

foreign activity

DNase, RNase, none detected

相关类别

General description

Agarose is commonly used in research for gel electrophoresis to separate DNA and RNA fragments. It is a natural polysaccharide isolated and purified from red algae. It has a linear structure consisting of alternating D-galactose and 3,6-anhydro-L-galactose units. When dissolved in solution, upon cooling, it forms a solid yet porous gel to use as a medium to separate nucleic acid fragments by size. When an electrical current is applied the charged molecules migrate through the gel.

Application

Agarose tablets can be used in research applications for:



  • Separation and analysis of nucleic acids by agarose gel electrophoresis, Northern Blot, or Southern Blot
  • Single Cell Gel Electrophoresis (SCGE), also known as comet assays
  • Hydrocolloid gels to study the structural influence of gels on the release of carbohydrates
  • Ouchterlony double immunodiffusion plates used to quantify antibodies and antigens
  • Radial immunodiffusion (RID)

Features and Benefits

  • Qualified for molecular biology applications
  • Free of endonucleases: DNase and RNase
  • Convenient, quick and easy to use
  • Low EEO and gelling temperature for routine analysis
  • High gel strength 1,200 g/cm2 (1% gel)
  • Separates DNA & RNA fragments 100bp - 23kb in 2% - 0.5% agarose gel, respectively.
  • Northern or Southern blotting, preferred for fragment sizes >1kb

存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

历史批次信息供参考:

分析证书(COA)

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