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STS0033

Agarose tablets

Name: Agarose tablets
Brand: SIGMA

Low EEO, For molecular biology (200 x 500mg tablets)

别名:

3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose LE, Agarose tablets

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关于此项目

化学文摘社编号:

9012-36-6

UNSPSC Code:

41105317

NACRES:

NB.21

MDL number:

MFCD00081294

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属性

biological source

algae (marine)

grade

Molecular Biology

form

tablet

impurities

≤10.0% moisture content

color

white

EEO

0.09-0.13 (-Mr)

transition temp

transition temp 34.5-37.5 °C

gel strength

≥1200 g/cm² (1% gel)

anion traces

sulfate (SO₄^2-): ≤0.15%

suitability

suitable for electrophoresis, suitable for molecular biology

foreign activity

DNase, RNase, none detected

SMILES string

O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

说明

General description

Agarose is commonly used in research for gel electrophoresis to separate DNA and RNA fragments. It is a natural polysaccharide isolated and purified from red algae. It has a linear structure consisting of alternating D-galactose and 3,6-anhydro-L-galactose units. When dissolved in solution, upon cooling, it forms a solid yet porous gel to use as a medium to separate nucleic acid fragments by size. When an electrical current is applied the charged molecules migrate through the gel.

Application

Agarose tablets can be used in research applications for:

Separation and analysis of nucleic acids by agarose gel electrophoresis, Northern Blot, or Southern Blot
Single Cell Gel Electrophoresis (SCGE), also known as comet assays
Hydrocolloid gels to study the structural influence of gels on the release of carbohydrates
Ouchterlony double immunodiffusion plates used to quantify antibodies and antigens
Radial immunodiffusion (RID)

Features and Benefits

Qualified for molecular biology applications
Free of endonucleases: DNase and RNase
Convenient, quick and easy to use
Low EEO and gelling temperature for routine analysis
High gel strength 1,200 g/cm2 (1% gel)
Separates DNA & RNA fragments 100bp - 23kb in 2% - 0.5% agarose gel, respectively.
Northern or Southern blotting, preferred for fragment sizes >1kb

存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c