T3568
TrypZean® 牛
recombinant, expressed in corn, lyophilized powder, ≥3,350 units/mg solid (USP)
别名:
胰蛋白酶 牛
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-650-8
MDL number:
Specific activity:
≥3,350 units/mg solid (USP)
Recombinant:
expressed in corn
recombinant
expressed in corn
form
lyophilized powder
specific activity
≥3,350 units/mg solid (USP)
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
cow ... TRYP8(282603)
General description
TrypZean® eliminates the introduction of animal source contaminants found in traditional bovine and porcine trypsins.
Application
Eliminates the introduction of animal source contaminants found in traditional bovine and porcine trypsins.
TrypZean® has been used in a study to assess the use of animal protein-free products for passaging adherent human adipose-derived stromal/stem cells. TrypZean® has also been used in a study to compare a recombinant trypsin with the porcine pancreatic extract on sperm used for the in vitro production of bovine embryos.
Biochem/physiol Actions
For dissociation of cultured cells.
Preparation Note
Manufactured by Applied Biotechnology Institute utilizing proprietary transgenic plant protein expression system.
Other Notes
查看更多关于 胰蛋白酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer
Legal Information
Trypzean is a registered trademark of ProdiGene, Inc.
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 1
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
常规特殊物品
此项目有
240 recombinant bovine trypsin made in maize inactivates bovine herpes virus-1 adsorbed to the bovine Zona Pellucida
Seidel, G., et al.
Reproduction, Fertility, and Development, 19, 236-236 (2006)
K J Mattson et al.
Theriogenology, 69(6), 724-727 (2008-02-19)
The objective was to compare the effects of treating bovine semen with two trypsin products (the porcine pancreas extract and a recombinant) and a control (no trypsin) on in vitro embryo production. Our hypothesis was that the trypsin treatments would
Use of animal protein-free products for passaging adherent human adipose-derived stromal/stem cells.
Pedro P Carvalho et al.
Cytotherapy, 13(5), 594-597 (2011-01-05)
Adherent adipose-derived stromal/stem cells (ASC) have been used in pre-clinical regenerative medical studies applied to a broad range of tissues with an ultimate goal of translating these findings to clinical safety and efficacy testing; however, many protocols passage the cells
Arun K Sharma et al.
Biotechnology advances, 27(6), 811-832 (2009-07-07)
In recent years, the use of plants as bioreactors has emerged as an exciting area of research and significant advances have created new opportunities. The driving forces behind the rapid growth of plant bioreactors include low production cost, product safety
Different methods of detaching adherent cells significantly affect the detection of TRAIL receptors.
Bo Zhang et al.
Tumori, 98(6), 800-803 (2013-02-08)
As a powerful technique allowing analysis of large numbers of cells, fluorescence-activated cell sorting (FACS) is used more and more widely. For FACS analysis, adherent cells are usually detached by trypsinization, followed by centrifugation and resuspension. However, trypsinization can cut
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