生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen 46 kDa
种属反应性
rat, mouse, human
浓度
~2 mg/mL
技术
western blot: 0.5-1 μg/mL using cell lysates of rat fibroblast Rat-1 and mouse fibroblast NIH3T3 cells
western blot: 2-4 μg/mL using cell lysates of human A431 cells
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... TSG101(7251)
mouse ... Tsg101(22088)
rat ... Tsg101(292925)
一般描述
肿瘤易感性101（TSG101）由定位于人类染色体11p15.1-15.2的基因编码。编码的蛋白质是催化失活的泛素E2变体（UEV）家族的成员。Tsg101的特征是在其N末端具有泛素E2变体（UEV）结构域，参与HIV-1出芽和液泡蛋白分选（VPS）途径。
免疫原
合成肽对应于位于人TSG101的C端的氨基酸254-272，与KLH偶联。该序列在大鼠和小鼠TSG101中相同。
应用
兔抗TSG101抗体可用于蛋白质印迹分析。
生化/生理作用
肿瘤易感性101（TSG101）涉及各种细胞功能，例如泛素化，转录调节，内体运输和细胞增殖。基因突变导致各种人类癌症。Tsg101在细胞生长、细胞存活以及胚胎和成人组织的正常功能中起着至关重要的作用。TSG101通过将p6晚期结构域与液泡蛋白分选机制连接来促进感染性人类免疫缺陷病毒（HIV-1）出芽。Tsg101是运输（ESCRT）机械所需的内体分选复合物的重要组成部分，有助于合成多囊泡体，主要是腔内囊泡的形成和分裂，因此对位在质膜上的受体的降解和再循环至关重要。Tsg101的表达受内在的自动调节机制和各种泛素连接酶催化的泛素化控制。
外形
溶于0.01 M磷酸盐缓冲液（pH 7.4）中，含有15 mM叠氮化钠。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Dihan Zhu et al.
Stem cell research & therapy, 11(1), 162-162 (2020-04-24)
M2 macrophages and exosomes from adipose-derived stem cells (ASCs) are both reported to promote angiogenesis. However, the possible synergistic effects between exogenous exosomes and endogenous M2 macrophages are poorly understood. Exosomes were isolated from conditioned medium of normoxic and hypoxic
J E Garrus et al.
Cell, 107(1), 55-65 (2001-10-12)
Like other enveloped viruses, HIV-1 uses cellular machinery to bud from infected cells. We now show that Tsg101 protein, which functions in vacuolar protein sorting (Vps), is required for HIV-1 budding. The UEV domain of Tsg101 binds to an essential
Olga Dolnik et al.
PLoS pathogens, 10(10), e1004463-e1004463 (2014-10-21)
Endosomal sorting complex required for transport (ESCRT) machinery supports the efficient budding of Marburg virus (MARV) and many other enveloped viruses. Interaction between components of the ESCRT machinery and viral proteins is predominantly mediated by short tetrapeptide motifs, known as
Precipitation with polyethylene glycol followed by washing and pelleting by ultracentrifugation enriches extracellular vesicles from tissue culture supernatants in small and large scales
Ludwig AK, et al.
Journal of Extracellular Vesicles, 7(1), 1528109-1528109 (2018)
James V McCann et al.
Scientific reports, 9(1), 16743-16743 (2019-11-16)
Extracellular vesicles (EVs) play important roles in tumor progression by altering immune surveillance, promoting vascular dysfunction, and priming distant sites for organotropic metastases. The miRNA expression patterns in circulating EVs are important diagnostic tools in cancer. However, multiple cell types
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