T6424
胰蛋白酶 来源于人类胰腺
salt-free, lyophilized powder, vial of ≥1,000 BAEE units
别名:
Serine Protease 1
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-650-8
MDL number:
Biological source:
human pancreas
产品名称
胰蛋白酶 来源于人类胰腺, salt-free, lyophilized powder, vial of ≥1,000 BAEE units
biological source
human pancreas
form
salt-free, lyophilized powder
packaging
vial of ≥1,000 BAEE units
storage temp.
−20°C
Quality Level
Gene Information
human ... PRSS1(5644), PRSS2(5645), PRSS3(5646)
Analysis Note
通过 E1%/280 确定的蛋白质。
Application
胰蛋白酶已被用于一项评估戊型肝炎病毒和人类星状病毒之间的相似性的研究。在一项研究中也使用了胰蛋白酶来表征培养原代成人上皮祖细胞或干细胞的一种独特技术。
General description
胰蛋白酶分子有两个结构域：一个与酶的活性位点和色氨酸残基有关；另一种与 8-苯胺萘-1-磺酸结合有关。
胰蛋白酶是一种丝氨酸蛋白酶。胰腺可产生前体胰蛋白酶原并将其分泌到肠中。肠粘膜中的肠激酶可将胰蛋白酶原转化为胰蛋白酶。
Other Notes
使用BAEE作为底物，一个BAEE单元将在pH7.6,25℃下产生每分钟0.001的A253。 反应体积= 3.2mL（1cm光程）。
查看更多关于 胰蛋白酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer
Preparation Note
至少 1,000 个 BAEE 单位/瓶
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 1
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
常规特殊物品
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实验方案
Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.
本方案适用于使用Nα-苯甲酰基-L-精氨酸乙酯（BAEE）作为底物测定胰蛋白酶活性。本方案是基于连续分光光度速率测定（A253，光程= 1cm）方案：
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