生物来源
bovine pancreas
质量水平
类型
Type V-S
表单
powder
比活
≥8,500 BAEE units/mg protein (biuret)
UniProt登记号
储存温度
−20°C
基因信息
cow ... TRYP8(282603)
一般描述
The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.
应用
Trypsin has been used in a study to assess the ontogeny of the endocrine pancreas in the fetal/newborn baboon. Trypsin digestion and hydrogen/deuterium exchange support the transition between inward- and outward-facing conformations during the catalytic cycle of the bacterial multidrug ATP-binding cassette transporter.
生化/生理作用
Lys-acetylated trypsin is less susceptible to autolysis than the native form.
制备说明
Prepared by the method of Labouesse, J. and Gervais, M., Eur. J. Biochem., 2, 215 (1967).
其他说明
One BAEE unit will produce a ΔA253 of 0.001 per min at pH 7.6 at 25° C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).
View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer.
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
11 - Combustible Solids
WGK
WGK 3
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
常规特殊物品
低风险生物材料
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Shin-Ichi Nakakita et al.
Methods in molecular biology (Clifton, N.J.), 2132, 567-583 (2020-04-20)
We describe a method to detect influenza virus using an evanescent-field-activated fluorescence scanner type glycan array and ELISA system. Neoglycoprotein was prepared by combination of organic chemistry and biomaterial preparation. These ligands were spotted on a glass plate or plastic
Nongluk Sriwilaijaroen et al.
Methods in molecular biology (Clifton, N.J.), 1200, 107-120 (2014-08-15)
The correlation between precise interactions of influenza A virus hemagglutinins with host cell surface glycans having terminal sialic acids and host range specificity has provoked the development of a high-throughput viral-receptor specificity assay. Here, we describe the use of the
Caroline Chupin et al.
Vaccines, 9(10) (2021-10-27)
The development of a live-attenuated vaccine (LAV) for the prevention of human metapneumovirus (HMPV) infection is often hampered by the lack of highly efficient and scalable cell-based production systems that support eventual global vaccine production. Avian cell lines cultivated in
R F Regan et al.
Journal of neurochemistry, 70(1), 77-85 (1998-01-09)
The effect of magnesium on the oxidative neuronal injury induced by hemoglobin was assessed in murine cortical cell cultures. Exposure to 5 microM hemoglobin in physiologic (1 mM) magnesium for 26 h resulted in the death of about one-half the
Shahid Mehmood et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(27), 10832-10836 (2012-06-20)
The study of membrane proteins remains a challenging task, and approaches to unravel their dynamics are scarce. Here, we applied hydrogen/deuterium exchange (HDX) coupled to mass spectrometry to probe the motions of a bacterial multidrug ATP-binding cassette (ABC) transporter, BmrA
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