Merck
CN

T8154

Sigma-Aldrich

台盼蓝 溶液

0.4%, liquid, sterile-filtered, suitable for cell culture

登录查看公司和协议定价

别名:
Blue cell viability dye
经验公式(希尔记法):
C34H24N6Na4O14S4
CAS号:
分子量:
960.81
Beilstein:
4360496
MDL编号:
PubChem化学物质编号:
NACRES:
NA.75

无菌性

sterile-filtered

质量水平

形式

liquid

储存条件

dry at room temperature

浓度

0.4%

technique(s)

cell culture | mammalian: suitable
tissue processing: suitable

application(s)

cell analysis

运输

ambient

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

正在寻找类似产品? Visit 产品对比指南

一般描述

台盼蓝(TB)是一种阴离子亲水性偶氮染料,仅能穿过死细胞的细胞膜,因此可将死组织/细胞染成蓝色。用于通过拒染法显微计数,区分活的和死的哺乳动物细胞。
台盼蓝是一种用于区分活细胞和死细胞的染料。它是一种不会被健康的活细胞所吸收但会对细胞膜受损的细胞进行染色的重要染料。这样,仅有死细胞会被计数。这种方法有时也被称为染料排除方法。

应用

台盼蓝溶液已用于:
  • 细胞活力检测,计数活细胞和死细胞
  • 在rescue实验中计数活细胞
  • 台盼蓝拒染计数法,测定增殖曲线
  • 配制生理盐水对照和脂多糖(LPS)溶液,确认LPS成功输注
  • 台盼蓝拒染实验,测定胃上皮细胞(GEC)活力
  • 通过血球计数板计数GEC细胞

制备说明

在 0.81% 氯化钠 和 0.06% 磷酸氢二钾中配制。

象形图

Health hazard

警示用语:

Danger

危险声明

危险分类

Carc. 1B

储存分类代码

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

为方便起见,与您过往购买产品相关的文件已保存在文档库中。

访问文档库

难以找到您所需的产品或批次号码?

在网站页面上,产品编号会附带包装尺寸/数量一起显示(例如:T1503-25G)。请确保 在“产品编号”字段中仅输入产品编号 (示例: T1503).

示例

T1503
货号
-
25G
包装规格/数量

其它示例:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

遇到问题?欢迎随时联系我们技术服务 寻求帮助

批号可以在产品标签上"批“ (Lot或Batch)字后面找到。

Aldrich 产品

  • 如果您查询到的批号为 TO09019TO 等,请输入去除前两位字母的批号:09019TO。

  • 如果您查询到的批号含有填充代码(例如05427ES-021),请输入去除填充代码-021的批号:05427ES。

  • 如果您查询到的批号含有填充代码(例如 STBB0728K9),请输入去除填充代码K9的批号:STBB0728。

未找到您寻找的产品?

部分情况下,可能未在线提供COA。如果搜索不到COA,可在线索取。

索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. Do the cells need to be washed to remove the culture media prior to staining with Product T8154, Trypan Blue solution?

    Trypan Blue has a greater affinity for serum proteins than for cellular protein. If the background is too dark, cells should be pelleted and resuspended in protein-free medium or salt solution prior to counting.

  3. Why is Trypan blue, Product T8154, used to determine cell viability?

    Trypan Blue is a vital stain recommended for use in estimating the proportion of viable cells in a population. The reactivity of this dye is based on the fact that the chromophore is negatively charged and does not react with the cell unless the membrane is damaged. Staining facilitates the visualization of cell morphology. Live (viable) cells do not take up the dye and dead (non-viable) cells do.

  4. Can Trypan blue, Product T8154, be used to discriminate between apoptotic and necrotic cells?

    Trypan blue will stain cells that have disrupted membranes.  It cannot differentiate between permeabilized membranes caused by apoptosis or necrosis.

  5. Trypan blue, Product T8154, contains a precipitate.  Is it still good to use?

    The trypan blue solution is still good to use.  It can be warmed to dissolve the precipitate or it can be filtered to remove the precipitate.

  6. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. Why are there red fibers in Product T8154, Trypan blue solution?

    A report of fibers in Product T8154, Trypan blue solution is sufficiently common almost to be a characteristic of the product. These fibers are a cosmetic fault rather than one of functionality.  The presence of fibers may be more pronounced following cold storage or shipping.  This product should be stored at room temperature. Filtration to remove the fibers will not affect the use of the product.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

The sensitivity of 5-formylcytosine to doxorubicin regardless of DNA damage
Selcen
Turkiye Entomoloji Dergisi (Turkish Journal of Entomology), 40 (2016)
Roya Khosravi-Far, Zahra Zakeri
Programmed Cell Death (2008)
Single cell isolation process with laser induced forward transfer
Yu Deng
Journal of Biological Engineering, 11(2) (2017)
Vital dyes in vitreoretinal surgery: chromovitrectomy
Meyer CH
Developments in Ophthalmology, 42 (2008)
S R Mishra et al.
Research in veterinary science, 108, 98-111 (2016-09-25)
The present study investigated the expression and localization of FGF and its functional receptors in the follicle of buffalo and the treatment of FGF2 on mRNA expression of CYP19A1 (aromatase), PCNA, and BAX (BCL-2 associated X protein) in cultured buffalo

商品

Cell counting protocol using a hemocytometer for monitoring cell viability. Discover other cell counting tools such as the Scepter™ 3.0 Cell Counter.

Lung organoids are valuable 3D models for human lung development and respiratory diseases. The 3dGRO™ differentiation protocol generates organoids from human iPSCs in 4 steps.

Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.

新型无血清、无异种人间充质干细胞(MSC)骨细胞分化培养基的开发。

查看所有结果

实验方案

Step-by-step protocol for ADCC assays using cryopreserved NK effector cells including tips and tricks, assay development, and data analysis methods.

Step-by-step stem cell culture protocols for human induced pluripotent stem cells (iPSCs) including ips cell thawing, expanding, freezing and characterizing.

有关间充质的信息,特别是间充质干细胞培养实验方案用于间充质干细胞(MSC)分离、扩增和分化的分步细胞培养方案。

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门