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Merck
CN

T8154

台盼蓝 溶液

0.4%, liquid, sterile-filtered, suitable for cell culture

别名:

Blue cell viability dye

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关于此项目

经验公式(希尔记法):
C34H24N6Na4O14S4
化学文摘社编号:
分子量:
960.81
UNSPSC Code:
12352207
NACRES:
NA.75
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
4360496
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sterility

sterile-filtered

Quality Level

form

liquid

storage condition

dry at room temperature

concentration

0.4%

technique(s)

cell culture | mammalian: suitable, tissue processing: suitable

application(s)

cell analysis

shipped in

ambient

storage temp.

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

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General description

台盼蓝是一种用于区分活细胞和死细胞的染料。它是一种不会被健康的活细胞所吸收但会对细胞膜受损的细胞进行染色的重要染料。这样,仅有死细胞会被计数。这种方法有时也被称为染料排除方法。
相同产品,但是规格为方便使用的冻干粉末形式,请见T6146

台盼蓝(TB)是一种阴离子亲水性偶氮染料,仅能穿过死细胞的细胞膜,因此可将死组织/细胞染成蓝色。用于通过拒染法显微计数,区分活的和死的哺乳动物细胞。

Application

台盼蓝溶液已用于:
  • 细胞活力检测,计数活细胞和死细胞
  • 在rescue实验中计数活细胞
  • 台盼蓝拒染计数法,测定增殖曲线
  • 配制生理盐水对照和脂多糖(LPS)溶液,确认LPS成功输注
  • 台盼蓝拒染实验,测定胃上皮细胞(GEC)活力
  • 通过血球计数板计数GEC细胞

Preparation Note

在 0.81% 氯化钠 和 0.06% 磷酸氢二钾中配制。

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Carc. 1B

存储类别

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


历史批次信息供参考:

分析证书(COA)

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全球贸易项目编号

货号GTIN
T8154-100ML04061838260109
T8154-1LSTAB04065272481816
T8154-20ML04061838260116

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