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Merck
CN

T9179

Sigma-Aldrich

TB培养基

EZMix powder microbial growth medium

别名:

TB media

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关于此项目

UNSPSC代码:
41106200
NACRES:
NA.85

主要文件

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等级

Molecular Biology

质量水平

200

描述

TB; quick dissolve, dust-free formulation

无菌性

non-sterile

表单

powder

技术

microbiological culture: suitable

应用

agriculture
food and beverages
microbiology

储存温度

room temp

适用性

nonselective for Escherichia coli
nonselective for coliforms

相关类别

一般描述

Terrific Broth是一个被高度引用的用于大肠杆菌培养的微生物生长培养基。 这种营养丰富的微生物肉汤含有甘油、肽、氨基酸、水溶性维生素和碳水化合物。

应用

适用于大肠杆菌菌株的非选择性培养，用于克隆、高产量生产 DNA 质粒和高产量生产重组蛋白。当添加适当的抗生素时，也适用于选择性培养。
使用了很棒的肉汤：
  • 作为在 pET28GST-LIC 中选择性生长表达 MLL SET（核原癌基因）结构域的大肠杆菌 BL21（DE3） V2RpRARE 的培养基
  • 培养含有表达 IL-8（interleukin-8） 质粒的 BL21（DE3） 细胞
  • 很棒的肉汤也适用于细菌悬浮液（枯草芽孢杆菌菌株 1085B）

特点和优势

很棒的 Broth EZMix 粉末提供：
  • 无尘颗粒，便于安全操作和更快混合
  • 一种预算较低的液体替代品
  • 改良配方（不含甘油）
  • 可通过大包装轻松放大规模

制备说明

将 24.1 g 粉末和 4 mL 甘油悬浮在足量的去离子水中，制成 500 mL 溶液。 在121 °C下高温灭菌15分钟以灭菌。 先冷却再添加其他成分，如抗生素（如果需要）。

其他说明

*请注意，某些产品规格以液体形式提供。 本品为粉末形式，并且这些规格反映了最终的重悬体积。

23.6g/L酵母提取物
11.8g/L 胰蛋白胨
9.4g/L K2HPO4
2.2g/L KH2PO4

法律信息

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

监管及禁止进口产品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000477990
0000477990
0000459950
0000459950
0000436318

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Which bacterial culture medium is the best choice for my application?

    Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production.  The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Optimizing antibody immobilization strategies for the construction of protein microarrays
Peluso P, et al.
Analytical Biochemistry, 312(2), 113-124 (2003)
Paul Peluso et al.
Analytical biochemistry, 312(2), 113-124 (2003-01-18)
Antibody microarrays have the potential to revolutionize protein expression profiling. The intensity of specific signal produced on a feature of such an array is related to the amount of analyte that is captured from the biological mixture by the immobilized
Eve Toulza et al.
Applied and environmental microbiology, 76(13), 4521-4529 (2010-05-04)
Understanding the conditions leading to harmful algal blooms, especially those produced by toxic dinoflagellate species, is important for environmental and health safety. In addition to investigations into the environmental conditions necessary for the formation of toxic blooms, we postulate that
Idan Tuval et al.
Proceedings of the National Academy of Sciences of the United States of America, 102(7), 2277-2282 (2005-02-09)
Aerobic bacteria often live in thin fluid layers near solid-air-water contact lines, in which the biology of chemotaxis, metabolism, and cell-cell signaling is intimately connected to the physics of buoyancy, diffusion, and mixing. Using the geometry of a sessile drop
Design of a fluorescent ligand targeting the S-adenosylmethionine binding site of the histone methyltransferase MLL1
Luan Y, et al.
Organic & Biomolecular Chemistry, 14(2), 631-638 (2016)
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实验方案

微生物生长实验方案

在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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