V8137
抗 V5 兔抗
IgG fraction of antiserum
别名:
兔抗V5
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关于此项目
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, IP, WB
Citations:
121
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
technique(s)
immunocytochemistry: 5 μg/mL using V5 tagged fusion protein in methanol-acetone fixed transiently transfected 293T cells, immunoprecipitation (IP): 5 μg/mL using V5 tagged fusion protein from extracts of transfected cells, western blot: 2.5 μg/mL using V5 tagged fusion protein expressed in whole extracts of transfected cells
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
General description
V5 是一种氨基共末端蛋白（amino coterminal protein），由 C 末端富含半胱氨酸的区域组成，类似于富含半胱氨酸的金属结合域。由副粘病毒 SV5 的“P”基因编码。
兔抗 V5 合成肽抗体与在转染的哺乳动物细胞中表达的 V5 标记的重组融合蛋白反应并从体外转化反应。
兔抗 V5 合成肽抗体与在转染的哺乳动物细胞中表达的 V5 标记的重组融合蛋白反应并从体外转化反应。
Application
兔体内产生的抗 V5 抗体用于：
- 免疫细胞化学
- 免疫沉淀
- 蛋白质印迹
Biochem/physiol Actions
识别 V5 Tag (GKPIPNPLLGLDST) 融合蛋白
Physical form
0.01 M 磷酸盐缓冲液 (pH 7.4) 中的溶液，含 15 mM 叠氮化钠
Preparation Note
可在 2-8°C储存最长一个月。若需延长储存时间，可将溶液分装并冷冻。不建议反复冻融。不建议在无霜冰箱中储存。如果长期储存时出现轻微浑浊，请在使用前通过离心澄清溶液。若工作稀释样品在12小时内未使用完，则应丢弃。
Disclaimer
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
低风险生物材料
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