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安全信息

WTA1

Sigma-Aldrich

TransPlex® Whole Transcriptome Amplification Kit

DNA polymerase separate.

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别名:
TransPlex®全转录组扩增试剂盒
UNSPSC代码:
41121800
NACRES:
NA.55

质量水平

200

技术

whole genome amplification: suitable

运输

wet ice

储存温度

−20°C

相关类别

一般描述

TransPlex®，一种全转录组扩增（WTA）方法，能够在4小时内，代表性地扩增出纳克级的总RNA而没有3′-偏差。 从组织、培养细胞、福尔马林固定样品或血清中所获得的微克级扩增产物适用于例如qPCR和微阵列分析等下游应用。

应用

TransPlex®全转录组扩增试剂盒已用于合成双链cDNA。还可用于cDNA扩增。
适于各种下游应用，包括：
  • qPCR
  • 微阵列分析
  • 克隆

特点和优势

  • 在4小时内对总RNA进行扩增，手动操作时间低于30分钟
  • 仅需5 ng起始材料，即可从总RNA扩增为极具代表性的文库
  • 以组织、培养的细胞、血清或来自福尔马林固定的石蜡包埋样品的降解RNA为原料，从完整的RNA可生成微克级的扩增产物

原理

WTA过程包括2个步骤。首先，使用由拟随机3′端和通用5′端组成的非自身互补引物对样品RNA进行逆转录。随着聚合作用的发生，被取代的单链将作为引物退火和延伸的新模板。所得OmniPlex cDNA文库由以通用末端序列为侧翼序列、含100-1000个碱基的随机重叠片段组成。随后，使用通用引物对文库进行PCR扩增，生成WTA产物。

法律信息

TransPlex is a registered trademark of Rubicon Genomics, Inc.

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • W4502Water, Nuclease-Free Water, for Molecular Biology化学品安全说明书
  • D7295Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent化学品安全说明书

象形图

Exclamation mark
GHS07

警示用语：

Warning

危险声明

H319

危险分类

Eye Irrit. 2

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the concentration of the dNTPs supplied with the TransPlex® Whole Transcriptome Amplification Kit, Product WTA1?

    The dNTP Mix (D6193) is supplied as 10 mM each.

  4. Is the TransPlex® Whole Transcriptome Amplification Kit, Product WTA1, also applicable for ribosomal RNA and/or small RNA amplification?

    Using the kit as is, Ribosomal RNA does appear to amplify. However, we have not quantitated how well it is amplified.

  5. What is the difference between WTA and WGA?   I use the TransPlex® Whole Transcriptome Amplification Kit, Product WTA1.

    The starting material for WTA is RNA, whereas for WGA it is genomic DNA.  After the RNA is reverse-transcribed into the DNA complement by WTA, the OmniPlex library is formed. This library is in turn amplified by PCR.

  6. Can TransPlex® Whole Transcriptome Amplification Kit, Product WTA1, be used to amplify archival fixed tissue or degraded samples?

    Yes, the WTA kit effectively amplifies degraded RNA, including formalin-fixed and paraffin-embedded samples.

  7. Can TransPlex® Whole Transcriptome Amplification Kit, Product WTA1, be used for downstream applications?

    Upon creation of a cDNA library, quantitative qPCR or microarray analysis may be performed

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Catarina Guimarães-Teixeira et al.
Journal of personalized medicine, 11(10) (2021-10-24)
(1) Background: Methylation of N6-adenosine (m6A) is the most abundant messenger RNA (mRNA) modification in eukaryotes. We assessed the expression profiles of m6A regulatory proteins in renal cell carcinoma (RCC) and their clinical relevance, namely, as potential biomarkers. (2) Methods:
Sa Xiao et al.
Virus research, 145(1), 80-91 (2009-06-23)
The complete genome sequence of avian paramyxovirus serotype 7 (APMV-7) prototype strain dove/Tennessee/4/75 was determined. The genome size is 15,480 nucleotides (nt) long and follows the "rule of six". The genome contains six non-overlapping genes in the order of 3'-N-P/V/W-M-F-HN-L-5'.
Munehiro Okamoto et al.
Scientific reports, 5, 8850-8850 (2015-03-07)
We discovered a lethal hemorrhagic syndrome arising from severe thrombocytopenia in Japanese macaques kept at the Primate Research Institute, Kyoto University. Extensive investigation identified that simian retrovirus type 4 (SRV-4) was the causative agent of the disease. SRV-4 had previously
Anne H Rowley et al.
The Journal of infectious diseases, 203(7), 1021-1030 (2011-03-16)
Intracytoplasmic inclusion bodies (ICI) have been identified in ciliated bronchial epithelium of Kawasaki disease (KD) patients using a synthetic antibody derived from acute KD arterial IgA plasma cells; ICI may derive from the KD etiologic agent. Acute KD bronchial epithelium
Sara Planamente et al.
Molecular microbiology, 86(5), 1085-1099 (2012-10-10)
GABA acts as an intercellular signal in eukaryotes and as an interspecies signal in host-microbe interactions. Structural characteristics of selective eukaryotic GABA receptors and bacterial GABA sensors are unknown. Here, we identified the selective GABA-binding protein, called Atu4243, in the
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实验方案

Integration of Sigma® TransPlex® with Agilent Microarray

TransPlex® kits' amplification products integrate seamlessly into existing Agilent workflows for microarray target expression analyses.

相关内容

Whole Transcriptome Amplification FAQs

Transplex Whole Transcriptome Amplification FAQs on topics including whole transcriptome steps, RNA source, including archival fixed tissue, library purification, quantitation of the product and downstream applications

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