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Merck
CN

Y1251

酵母氮源基础，不含氨基酸和硫酸铵

NutriSelect® Basic, powder, for fungi, poly bottle of 500 g, poly bottle of 1 kg

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关于此项目

NACRES:
NA.85
UNSPSC Code:
41171606
Manufacturer/tradename:
NutriSelect® Basic
Form:
powder
Suitability:
Candida spp., Pichia spp., Saccharomyces spp., Zygosaccharomyces spp., fungi, molds, yeasts
Application(s):
clinical testing
food and beverages
life science and biopharma
microbiology

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sterility

non-sterile

form

powder

packaging

poly bottle of 1 kg, poly bottle of 500 g

manufacturer/tradename

NutriSelect® Basic

solubility

water: soluble 67 mg/mL

application(s)

clinical testing
food and beverages
life science and biopharma
microbiology

suitability

Candida spp., Pichia spp., Saccharomyces spp., Zygosaccharomyces spp., fungi, molds, yeasts

Quality Level

200

相关类别

General description

酵母氮源基础/Yeast Nitrogen Base（不含氨基酸和硫酸铵）推荐用于根据酵母的碳源和氮源需求进行菌种分类。培养基包含酵母生长必需的所有营养素，但不含氨基酸、组氨酸、甲硫氨酸和色氨酸，以及硫酸铵。根据《临床微生物学手册》所述，培养时还需要添加氮源和碳源。

Application

酵母氮源基础/YNB（不含氨基酸和硫酸铵）用于通过碳源和氮源利用对酵母进行分类。除氨基酸、氮源和碳水化合物之外，包含酵母生长的所有必需营养素。需要添加氮源和碳源。

Preparation Note

  • 将1.7 g不含氨基酸和硫酸铵的酵母氮源混悬于冷却的100 mL蒸馏水中，制得10×储备溶液。包含所需的氮和碳源。
  • 必要时可加温溶解，然后过滤灭菌。
  • 将10×储备溶液储存于2-8°C。使用时，在无菌条件下用无菌蒸馏水1:10稀释。
成分
生物素，2 μg/L
泛酸钙，400 μg/L
叶酸，2 μg/L
烟酸, 400 μg/L
对氨基苯甲酸，200 μg/L
盐酸吡哆醇，400 μg/L
核黄素，200 μg/L
盐酸硫胺素，400 μg/L
肌醇，2 mg/L
硼酸，500 μg/L
硫酸铜，40 μg/L
碘化钾，100 μg/L
氯化铁，200 μg/L
硫酸锰，400 μg/L
钼酸钠，200 μg/L
硫酸锌，400 μg/L
磷酸二氢钾，1 g/L
硫酸镁，0.5 g/L
氯化钠，0.1 g/L
氯化钙，0.1 g/L

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

我们现提供两种类型的培养基：GranuCult®优质颗粒状培养基和NutriSelect®高性价比粉末状培养基，满足用户不同层次的需求。商标后的品级称号basic（基础）、plus（加强）或prime（特级）代表产品的不同质控水平，从基础的质控到标准质控加强版到全面合规的特级质控水平。

仅试剂盒组分

产品编号
说明
  • KH2PO4 1 g/L
  • MgSO4 .5 g/L
  • NaCl .1 g/L
  • CaCl2 .1 g/L
  • Inositol 2 mg/L

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000524675
0000524675
0000432594
0000432594
0000478747

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Michael Mülleder et al.
Cell, 167(2), 553-565 (2016-10-04)
Genome-metabolism interactions enable cell growth. To probe the extent of these interactions and delineate their functional contributions, we quantified the Saccharomyces amino acid metabolome and its response to systematic gene deletion. Over one-third of coding genes, in particular those important for chromatin dynamics
Ligia Acosta-Sampson et al.
The Journal of biological chemistry, 292(48), 19610-19627 (2017-10-04)
Targeting of most integral membrane proteins to the endoplasmic reticulum is controlled by the signal recognition particle, which recognizes a hydrophobic signal sequence near the protein N terminus. Proper folding of these proteins is monitored by the unfolded protein response
Xin Li et al.
eLife, 4 (2015-02-04)
Sustainable biofuel production from renewable biomass will require the efficient and complete use of all abundant sugars in the plant cell wall. Using the cellulolytic fungus Neurospora crassa as a model, we identified a xylodextrin transport and consumption pathway required
Owen W Ryan et al.
eLife, 3 (2014-08-21)
The directed evolution of biomolecules to improve or change their activity is central to many engineering and synthetic biology efforts. However, selecting improved variants from gene libraries in living cells requires plasmid expression systems that suffer from variable copy number
Richard S Marshall et al.
eLife, 7 (2018-04-07)
26S proteasome abundance is tightly regulated at multiple levels, including the elimination of excess or inactive particles by autophagy. In yeast, this proteaphagy occurs upon nitrogen starvation but not carbon starvation, which instead stimulates the rapid sequestration of proteasomes into
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商品

Culture Method for Beer Quality Control

Traditional methods are based morphology, staining methods, enzyme reactions (metabolism) and diverse media.

实验方案

Yeast Growth Methods

Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.

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