产品名称
酵母氮源基础，不含氨基酸和硫酸铵, NutriSelect® Basic, powder, for fungi, poly bottle of 500 g, poly bottle of 1 kg
sterility
non-sterile
form
powder
packaging
poly bottle of 1 kg
poly bottle of 500 g
manufacturer/tradename
NutriSelect® Basic
solubility
water: soluble 67 mg/mL
application(s)
clinical testing
food and beverages
life science and biopharma
microbiology
suitability
Candida spp.
Pichia spp.
Saccharomyces spp.
Zygosaccharomyces spp.
fungi
molds
yeasts
Quality Level
Application
酵母氮源基础/YNB（不含氨基酸和硫酸铵）用于通过碳源和氮源利用对酵母进行分类。除氨基酸、氮源和碳水化合物之外，包含酵母生长的所有必需营养素。需要添加氮源和碳源。
Disclaimer
我们现提供两种类型的培养基：GranuCult®优质颗粒状培养基和NutriSelect®高性价比粉末状培养基，满足用户不同层次的需求。商标后的品级称号basic（基础）、plus（加强）或prime（特级）代表产品的不同质控水平，从基础的质控到标准质控加强版到全面合规的特级质控水平。
General description
酵母氮源基础/Yeast Nitrogen Base（不含氨基酸和硫酸铵）推荐用于根据酵母的碳源和氮源需求进行菌种分类。培养基包含酵母生长必需的所有营养素，但不含氨基酸、组氨酸、甲硫氨酸和色氨酸，以及硫酸铵。根据《临床微生物学手册》所述，培养时还需要添加氮源和碳源。
Preparation Note
- 将1.7 g不含氨基酸和硫酸铵的酵母氮源混悬于冷却的100 mL蒸馏水中，制得10×储备溶液。包含所需的氮和碳源。
- 必要时可加温溶解，然后过滤灭菌。
- 将10×储备溶液储存于2-8°C。使用时，在无菌条件下用无菌蒸馏水1:10稀释。
成分
生物素，2 μg/L
泛酸钙，400 μg/L
叶酸，2 μg/L
烟酸, 400 μg/L
对氨基苯甲酸，200 μg/L
盐酸吡哆醇，400 μg/L
核黄素，200 μg/L
盐酸硫胺素，400 μg/L
肌醇，2 mg/L
硼酸，500 μg/L
硫酸铜，40 μg/L
碘化钾，100 μg/L
氯化铁，200 μg/L
硫酸锰，400 μg/L
钼酸钠，200 μg/L
硫酸锌，400 μg/L
磷酸二氢钾，1 g/L
硫酸镁，0.5 g/L
氯化钠，0.1 g/L
氯化钙，0.1 g/L
生物素，2 μg/L
泛酸钙，400 μg/L
叶酸，2 μg/L
烟酸, 400 μg/L
对氨基苯甲酸，200 μg/L
盐酸吡哆醇，400 μg/L
核黄素，200 μg/L
盐酸硫胺素，400 μg/L
肌醇，2 mg/L
硼酸，500 μg/L
硫酸铜，40 μg/L
碘化钾，100 μg/L
氯化铁，200 μg/L
硫酸锰，400 μg/L
钼酸钠，200 μg/L
硫酸锌，400 μg/L
磷酸二氢钾，1 g/L
硫酸镁，0.5 g/L
氯化钠，0.1 g/L
氯化钙，0.1 g/L
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Ligia Acosta-Sampson et al.
The Journal of biological chemistry, 292(48), 19610-19627 (2017-10-04)
Targeting of most integral membrane proteins to the endoplasmic reticulum is controlled by the signal recognition particle, which recognizes a hydrophobic signal sequence near the protein N terminus. Proper folding of these proteins is monitored by the unfolded protein response
Xin Li et al.
eLife, 4 (2015-02-04)
Sustainable biofuel production from renewable biomass will require the efficient and complete use of all abundant sugars in the plant cell wall. Using the cellulolytic fungus Neurospora crassa as a model, we identified a xylodextrin transport and consumption pathway required
Owen W Ryan et al.
eLife, 3 (2014-08-21)
The directed evolution of biomolecules to improve or change their activity is central to many engineering and synthetic biology efforts. However, selecting improved variants from gene libraries in living cells requires plasmid expression systems that suffer from variable copy number
Michael Mülleder et al.
Cell, 167(2), 553-565 (2016-10-04)
Genome-metabolism interactions enable cell growth. To probe the extent of these interactions and delineate their functional contributions, we quantified the Saccharomyces amino acid metabolome and its response to systematic gene deletion. Over one-third of coding genes, in particular those important for chromatin dynamics
Sviatlana Shashkova et al.
Bio-protocol, 8(2), e2710-e2710 (2018-02-13)
Single-molecule fluorescence microscopy enables unrivaled sub-cellular quantitation of genomically encoded fusions of native proteins with fluorescent protein reporters. Fluorescent proteins must undergo in vivo maturation after expression before they become photoactive. Maturation effects must be quantified during single-molecule analysis. Here
商品
Traditional methods are based morphology, staining methods, enzyme reactions (metabolism) and diverse media.
实验方案
Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.
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