ZMS1193
Anti-Trop2 Antibody, clone 2G10 ZooMAb® Mouse Monoclonal
recombinant, expressed in HEK 293 cells
别名:
Anti-CD43 Antibody, clone 1O6 ZooMAb® Rabbit Monoclonal, Cell surface glycoprotein Trop-2, Membrane component chromosome 1 surface marker 1, Pancreatic carcinoma marker protein GA733-1, Tumor-associated calcium signal transducer 2
产品名称
Anti-Trop2 Antibody, clone 2G10 ZooMAb® Mouse Monoclonal, recombinant, expressed in HEK 293 cells
biological source
mouse
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
2G10, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 35.71 kDa
purified by
using protein G
species reactivity
human
packaging
antibody small pack of 25
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
ELISA: suitable
affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable
isotype
IgG2aκ
epitope sequence
Extracellular domain
Protein ID accession no.
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
Quality Level
Gene Information
human ... TACSTD2(4070)
General description
Tumor-associated calcium signal transducer 2 (UniProt: P09758; also known as Cell surface glycoprotein Trop-2, Trophoblast antigen 2, Membrane component chromosome 1 surface marker 1, Pancreatic carcinoma marker protein GA733-1) is encoded by the TACSTD2 (also known as GA733-1, M1S1, TROP2) gene (Gene ID: 4070) in human. Trop2 is a single-pass type 1 membrane glycoprotein that is synthesized with a signal peptide (aa 1-26), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 27-274), a transmembrane domain (aa 275-297), and a cytoplasmic domain (aa 298-323). Its expression is observed in placenta and is highly expressed in many cancers where it plays a role in tumor progression. About 85% of all solid cancers display increased expression of Trop2. Its expression is also reported in stem cells in various tissues. TROP2 expression in cancer cells has been correlated with drug resistance. Upregulation of Trop2 is reported to drive the e
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Application
Quality Control Testing
Evaluated by Western Blotting with recombinant fragment of Human Trop2.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected His- and hFc-tagged recombinant fragment of Human Trop2.
Tested Applications
Flow Cytometry Analysis: 1 µg from a representative lot detected Trop2 in one million HaCaT cells.
Enzyme Immunoassay (ELISA) Analysis: A serial of dilutions from a representative lot detected His-and hFc-tagged recombinant fragment of Human Trop2.
Affinity Binding Assay: A representative lot of this antibody bound His and hFc-tagged recombinant fragment of Human Trop2 with a KD of 1.5 x 10-6 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 filution from a representative lot detected Trop2 in HaCaT cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting with recombinant fragment of Human Trop2.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected His- and hFc-tagged recombinant fragment of Human Trop2.
Tested Applications
Flow Cytometry Analysis: 1 µg from a representative lot detected Trop2 in one million HaCaT cells.
Enzyme Immunoassay (ELISA) Analysis: A serial of dilutions from a representative lot detected His-and hFc-tagged recombinant fragment of Human Trop2.
Affinity Binding Assay: A representative lot of this antibody bound His and hFc-tagged recombinant fragment of Human Trop2 with a KD of 1.5 x 10-6 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 filution from a representative lot detected Trop2 in HaCaT cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 2G10 is a ZooMAb® mouse recombinant monoclonal antibody that specifically detects Tumor-associated calcium signal transducer 2 (Trop2).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
His and hFc-tagged recombinant protein corresponding to the extracellular domain of human Tumor-associated calcium signal transducer 2 (Trop2)
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
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