Anti-Aurora B Antibody, clone 7C4, ZooMAb^® Rabbit Monoclonal

Anti-Aurora B, clone 7C4 ZooMAb , Cat. No. ZRB1149, is a highly specific rabbit recombinant monoclonal antibody that targets Aurora B and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Aurora B in C6 cell lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Aurora B in HeLa, A431, HUVEC and NIH 3T3 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100-1,000 dilution from a representative lot detected Aurora B in human tonsil and mouse spleen tissue sections.

Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.

Clone 7C4 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Aurora Kinase B. It targets an epitope within the first 19 amino acids from the N-terminal region.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Aurora kinase B (UniProt: Q96GD4; also known as EC:2.7.11.1, Aurora 1, Aurora- and IPL1-like midbody-associated protein 1, AIM-1, Aurora/IPL1-related kinase 2, ARK-2, Aurora-related kinase 2, STK-1, Serine/threonine-protein kinase 12, Serine/threonine-protein kinase 5, Serine/threonine-protein kinase aurora-B) is encoded by the AURKB (also known as AIK2, AIM1, AIRK2, ARK2, STK1, STK12, STK5) gene (Gene ID: 9212) in human. Aurora Kinase B is a serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. It is a key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Aurora kinase B phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP and phosphorylation of INCENP leads to increased Aurora kinase B activity. Five isoforms of Aurora Kinase are reported that are produced by alternative splicing. Its expression is shown to be cell-cycle regulated, with low expression in G1/S phase and an increase during G2 and M phase. Following M phase its expression declines. Cancer cells display high expression of Aurora kinase B during M phase. Disruption in regulation of Aurora kinase B causes perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis. High level expression is observed in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Substitution of Lysine 106 with arginine leads to loss of its kinase activity and severely impairs mitotic progression. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

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KLH-conjugated linear peptide corresponding to the first 19 amino acids from the N-terminal region of human Aurora kinase B.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany