Caspase-8 (UniProt: O89110; also known as EC: 3.4.22.61, CASP-8, CASP8, MCH5, FLICE, MACH) is encoded by the Casp8 gene (Gene ID: 12370) in murine species. Caspase-8 is a cysteine protease that serves as a molecular switch for apoptosis, necroptosis, and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood. It is an initiator caspase that plays a major role in the extrinsic apoptotic signaling pathway via death receptors. It is expressed in wide variety of tissues and its highest expression has been reported in spleen, thymus, lung, liver, and kidney. Low levels of expression are observed in heart, brain, testis, and skeletal muscle. Caspase-8 is produced with pro-peptides (aa 1-218 and 377-387) that are cleaved under appropriate signals to produce a p20 (aa 219-376) and a p10 (aa 388-480) subunits that heterotetramerize to form active caspase-8 with p20 and two p10 subunits. Generation of the subunits requires association with the death-inducing signaling complex (DISC). Activated caspase 8 is subsequently released to the cytosol where it cleaves downstream effector caspases. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Newton, K., et al. (2019). Nature. 574(7778); 428-431; Fritsch, M., et al. (2019). Nature. 575(7784); 683-687).

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 23 amino acids from the cleavage site within the C-terminal half of mouse Caspase-8.

Quality Control Testing

Evaluated by Western Blotting in HeLa treated with Staurosporin.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Cleaved Caspase-8 in lysates from overnight serum starved HeLa cells treated with Staurosporin (0.5 M; 6 h).

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Cleaved Caspase-8 in lysates from NIH3T3 cells treated with Etoposide (100 nM; 2 h).

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Cleaved Caspase-8 in Mouse brain tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Cleaved Caspase-8 in NIH3T3 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 1K3 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects cleaved form of Caspase-8. It targets an epitope within 23 amino acids from the cleavage site within the C-terminal half.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.