Anti-RABL3 Antibody, clone 1C2 ZooMAb^® Rabbit Monoclonal

Rab-like protein 3 (UniProt: Q5HYI8; also known as RABL3) is encoded by the RABL3 gene (Gene ID: 285282) in human. RABL3 is a Rab-like small GTPase (2) that is highly conserved in mammalian species. It is required for KRAS signaling regulation and modulation of cell proliferation. It serves as a regulator of KRAS prenylation, and probably prenylation of other small GTPases. It is reported to be essential for lymphocyte development and function but is not for myeloid cell development. It is also reported to be essential for embryonic development and null mutations in RABL3 gene in mice result in embryonic lethality. Overexpression of RABL3 is known to enhance cell proliferation and motility, whereas its knockdown increased apoptosis in breast cancer cell lines. Mutations in RABL3 gene have been linked to increased susceptibility of exocrine and endocrine pancreatic cancers This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zhong, X., et al. (2020). Proc. Natl. Acad. Sci. USA. 117(15); 8563-8572; Li, Q., et al. (2010). Oncol. Rep. 24(2); 433-440).

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal half of human Rab-like protein 3 (RABL3).

Quality Control Testing

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RABL3 in MCF-7 cell lysate.

Tested applications

Affinity Binding Assay: A representative lot of this antibody bound RABL3 peptide with a KD of 1.0 x 10-12 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected RABL3 in MCF-7 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected RABL3 in human kidney tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RABL3 in MCF-7 cell lysate.

Clone 1C2 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Rab-like protein 3 (RABL3). It targets an epitope within 19 amino acids from the N-terminal half.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.