Anti-WWP2 Antibody, clone 1C18 ZooMAb^® Rabbit Monoclonal

NEDD4-like E3 ubiquitin-protein ligase WWP2 (UniProt: O00308; also known as EC:2.3.2.26, Atrophin-1-interacting protein 2, AIP2, HECT-type E3 ubiquitin transferase WWP2, WW domain-containing protein 2) is encoded by the WWP2 gene (Gene ID: 11960) in human. WWP2 is a member of the C2-WW-HECT family (NEDD4 family) of the E3 ubiquitin ligases. It accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It is highly expressed in undifferentiated embryonic stem cells and its expression is reduced after embryoid body formation. It is detected in heart, throughout the brain, placenta, lung, liver, muscle, kidney, and pancreas. It is also detected in spleen and peripheral blood leukocytes. WWP2 has four WW domains that mediate its interaction with PPxY motif-containing proteins. It also has a C2 domain (aa 1-117) that is involved in autoinhibition of its catalytic activity by interacting with the HECT domain (aa 536-870). WWP2 is reported to regulate the pro-fibrotic gene network in primary cardiac fibroblasts and any defects in this gene can lead to pathologic cardiac fibrosis. It is also reported to regulate the nucleo-cytoplasmic shuttling and TGFβ-signal transduction activity of SMAD2. In untreated cardiac fibroblasts, it is present mainly in the cytoplasm, however, upon TGFβ1 stimulation it is localized predominantly in the nucleus. WWP2 is also serves as a physiological ubiquitin ligase for PTEN and promotes PTEN degradation under physiological conditions. WWP2 knockout mice exhibited reduced body size, elevated PTEN protein levels and antagonizes PI-3 kinase/Akt signaling. Four isoforms of WWP2 have been described that are produced by alternative splicing. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Chen, H., et al. (2019). Nat. commun. 10: Article 3616; Li, H., et al. (2018). J. Biol. Chem. 293(23); 8886-8899).

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

His-tagged recombinant fragment corresponding to 172 amino acids from the N-terminal half of human NEDD4-like E3 ubiquitin-protein ligase WWP2.

Quality Control Testing

Evaluated by Western Blotting in C2C12 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected WWP2 in C2C12 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected WWP2 in C6 cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected WWP2 in human kidney tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound recombinant WWP2 protein fragment with a KD of 3.0 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in C2C12 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected WWP2 in C2C12 cell lysate.

Clone 1C18 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects NEDD4-like E3 ubiquitin-protein ligase WWP2. It targets an epitope within the N-terminal half.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.