Anti-HIF-2α/EPAS1 Antibody, clone 2L21 ZooMAb^® Rabbit Monoclonal

Endothelial PAS domain-containing protein 1 (UniProt: Q99814; also known as EPAS-1, Basic-helix-loop-helix-PAS protein MOP2, Class E basic helix-loop-helix protein 73, bHLHe73, HIF-1-alpha-like factor, HLF, Hypoxia-inducible factor 2-alpha, HIF-2-alpha, HIF2-alpha, Member of PAS protein 2, PAS domain-containing protein 2) is encoded by the EPAS1 (also known as BHLHE73, HIF2A, MOP2, PASD2) gene (Gene ID: 2034) in human. HIF-2 is a transcription factor that is involved in the induction of oxygen regulated genes. It displays about 48% identity to HIF-1 and is widely expressed with high expression observed in endothelial cells, placenta, lung, and heart. Its levels are strongly induced by hypoxic conditions. HIF-2 dimerizes with ARNT, via amino acid sequence 171-192, and this heterodimer binds to core DNA sequence 5′-TACGTG-3′ within the hypoxia response element (HRE) of target gene promoters. Its DNA-binding region is localized to amino acids 26-53. It has two PAS (Per-Arnt-Sim) dom

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ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal half of human HIF-2 /EPAS1.

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected HIF-2 /EPAS1 in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected His/ABP-tagged recombinant fragment of Human HIF-2 /EPAS1.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected HIF-2 /EPAS1 in HepG2 cells.

Affinity Binding Assay: A representative lot of this antibody bound HIF-2 /EPAS1 peptide with a KD of 1.1 x 10-8 in an affinity binding assay.

Enzyme Immunoassay (ELISA) Analysis: Serial of dilutions from a representative lot detected His/ABP-tagged recombinant fragment of Human HIF-2 /EPAS1.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 2L21 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects HIF-2 / EPAS1. It targets an epitope within 22 amino acids from the C-terminal half.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.