ZRB2404
Anti-RelB Antibody, clone 1M14 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
别名:
Anti-Trop2 Antibody, clone 2G10 ZooMAb® Mouse Monoclonal, I-Rel, Transcription factor RelB
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关于此项目
NACRES:
NA.43
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
1M14, recombinant monoclonal
Application:
ELISA
affinity binding assay
immunofluorescence
immunohistochemistry
western blot
affinity binding assay
immunofluorescence
immunohistochemistry
western blot
Species reactivity:
human, mouse
Citations:
Technique(s):
ELISA: suitable
affinity binding assay: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
affinity binding assay: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
Uniprot accession no.:
产品名称
Anti-RelB Antibody, clone 1M14 ZooMAb® Rabbit Monoclonal, recombinant, expressed in HEK 293 cells
biological source
rabbit
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
1M14, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 62.13 kDa
observed mol wt ~75 kDa
purified by
using Protein A
species reactivity
human, mouse
packaging
antibody small pack of 25
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
ELISA: suitable
affinity binding assay: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
epitope sequence
C-terminal half
Protein ID accession no.
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
Quality Level
Gene Information
human ... RELB(5971)
Application
Quality Control Testing
Evaluated by Western Blotting in RAW264.7 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RelB in RAW264.7 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected RelB in lysate from Raji and Daudi cells and Human tonsil tissue and T7-tagged recombinant fragment from Human RelB protein.
Enzyme Immunoassay (ELISA) Analysis: Serial dilutions from a representative lot detected T7-tagged recombinant fragment of Human RelB protein.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected RelB in Human tonsil tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected RelB in Human tonsil tissue sections.
Affinity Binding Assay Analysis: A representative lot of this antibody bound RelB peptide with a KD of 6.1 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in RAW264.7 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RelB in RAW264.7 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected RelB in lysate from Raji and Daudi cells and Human tonsil tissue and T7-tagged recombinant fragment from Human RelB protein.
Enzyme Immunoassay (ELISA) Analysis: Serial dilutions from a representative lot detected T7-tagged recombinant fragment of Human RelB protein.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected RelB in Human tonsil tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected RelB in Human tonsil tissue sections.
Affinity Binding Assay Analysis: A representative lot of this antibody bound RelB peptide with a KD of 6.1 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1M14 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects RelB. It targets an epitope within 22 amino acids from the C-terminal half.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal half of human RelB.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
11 - Combustible Solids
wgk
WGK 1
法规信息
常规特殊物品
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