Growth factor receptor-bound protein 2 (UniProt: P62993; also known as Adapter protein GRB2, Protein Ash, SH2/SH3 adapter GRB2) is encoded by the GRB2 (also known as ASH) gene (Gene ID: 2885) in human. GRB2 is a signaling adaptor that is ubiquitously expressed and interacts with numerous receptors and intracellular signaling molecules. It contains one central SH2 domain flanked by two SH3 domains and is broadly expressed in many tissues and is shown to be essential for embryonic development and multiple cellular functions. It is a major mediator in Ras-MAPK activation pathway induced by numerous receptors because of its association with son of sevenless (SoS). GRB2 plays a role in early T cell development  and is shown to be a positive regulator of TCR signaling. It does not directly affect the activities of Ras and Erk1/2; however, it amplifies the activity of Lck, a tyrosine kinase that functions at the top of the TCR signaling cascade. It exists in a constitutive equilibrium between monomeric and dimeric states. The monomeric form is capable of binding to SoS and upregulating MAP kinase signaling, and the dimeric state is inhibitory to this process. Phosphorylation of tyrosine 160 or binding of a tyrosylphosphate-containing ligand to the SH2 domain of Grb2, results in dimer dissociation. Phosphorylation of tyrosine 160 is readily detectable in the malignant prostate, colon. and breast cancers.  Two isoforms of GRB2 have been described that are produced by alternative splicing. Isoform 2 acts as a dominant negative protein that suppresses proliferative signals, may trigger active programmed cell death. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals. (Ref.: Ahmed, Z., et al. (2015). Nat. Commun. 6; Article7354; Jang, IK., et al. (2011). Proc. Natl. Acad. Sci. USA. 108 (19); 7926-7931; Fath, I., et al. (1994). Science. 264(5161); 971-974; Lowenstein, EJ., et al. (1992). Cell. 70(3); 431-442).

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 1J2 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Growth factor receptor-bound protein 2 (GRB2). It targets an epitope within 15 amino acids from the C-terminal half.

KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal half of human Growth factor receptor-bound protein 2 (GRB2).

Quality Control Testing

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GRB2 in HEK293 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GRB2 in HeLa, PC12, and RAW264.7 cell lysates.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GRB2 in HEK293 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GRB2 in Human tonsil tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Affinity is a trademark of Mine Safety Appliances Co.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.