NAD-dependent protein deacetylase sirtuin-2 (UniProt: Q8IXJ6; also known as EC:2.3.1.286, NAD-dependent protein defatty-acylase sirtuin-2, Regulatory protein SIR2 homolog 2, SIR2-like protein 2) is encoded by the SIRT2 (also known as SIR2L, SIR2L2) gene (Gene ID: 22933) in human. SIRT2 is a NAD-dependent protein deacetylase that deacetylates internal lysines on histone and -tubulin as well as several key transcription factors. Its deacetylase domain is localized in amino acids 57-338 and its nuclear export signal motif is in amino acids 41-51. SIRT2 participates in the modulation of multiple and diverse biological processes, including cell cycle control, genomic integrity, microtubule dynamics, cell differentiation, metabolic networks, and autophagy. It serves as an antephase checkpoint that prevents early mitotic entry in response to microtubule stress agents and control of cell cycle progression and genomic stability. It is reported to specifically deacetylate histone H4 at lysine 16 (H4K16ac) between the G2/M transition and metaphase enabling H4K20me1 deposition by KMT5A leading to ulterior levels of H4K20me2 and H4K20me3 deposition throughout cell cycle, and mitotic S-phase progression. SIRT2 is phosphorylated at serine 368 by CDK1/cyclin B at the G2/M transition and this phosphorylation event regulates the delay in cell-cycle progression. However, phosphorylation at serine 368 by Cdk2/cyclin E complex inactivates SIRT2-mediated -tubulin deacetylation, and negatively regulates cell adhesion, cell migration, and neurite outgrowth during neuronal differentiation. Phosphorylation at serine 331 (isoforms 2 and 3) by Cdk2/cyclin E and Cdk2/cyclin A is reported to inhibit the catalytic activity of SIRT2. SIRT2 levels are up-regulated in response to low levels of glucose and anoxia-reoxygenation stress and down-regulated in response to high levels of glucose. It regulates blood glucose homeostasis by deacetylating and stabilizing phosphoenolpyruvate carboxykinase. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Pandithage, R., et al. (2008). J. Cell Biol. 180(5); 915-929; North, BJ., and Verdin, E. (2007). J. Biol. Chem. 282(27); 19546-19555; North, BJ., et al. (2003). Mol. Cell. 11(2); 437-444; Dryden SC., et al. (2003). Mol. Cell Biol. 23(9); 173-185).

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal region of human SIRT2 (Sirtuin-2).

Quality Control Testing

Evaluated by Western Blotting in Human brain tissue lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected SIRT2 in Human brain tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected SIRT2 in HEK293 cell lysate.

Affinity Binding Assay: A representative lot of this antibody bound SIRT2 peptide with a KD of 3.0 x 10-8 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected SIRT2 in Human cerebral cortex tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 1D4 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects SIRT2. It targets an epitope within 16 amino acids from the C-terminal region.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.