Anti-Collagen IV α2 Antibody, clone 1D9 ZooMAb^® Rabbit Monoclonal

Quality Control Testing

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Collagen IV 2 in HEK293 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Collagen IV 2 in Human kidney and Human placenta tissue lysates.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Collagen IV 2 in A431 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Collagen IV 2 in Human kidney tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound Collagen IV 2 peptide with a KD of 1.0 x 10-12 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 1D9 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects collagen Type IV 2. It targets an epitope within 18 amino acids from the N-terminal half.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Collagen alpha-2(IV) chain (UniProt: P08572) is encoded by the COL4A2 gene (Gene ID: 1284) in human. Collagen, the most abundant protein in the body is predominantly synthesized by fibroblasts; however, epithelial cells may also synthesize small amounts of collagen. The collagen super family includes over 20 different types of collagens with at least 38 distinct polypeptide chains. Of these types I, II, and III are the most abundant and are involved in the formation of fibrils. Type IV collagen forms a two-dimensional reticulum and is a major component of the basal lamina. It is a network-forming collagen that underlies epithelial and endothelial cells and functions as a barrier between tissue compartments. Type IV collagen has many binding partners and forms the backbone of the basement membrane. The type IV collagen family of proteins comprises six isomeric chains, designated 1(IV) to 6(IV), each of which can form a triple helix structure with 2 other chains to generate type IV collagen network. Collagen IV 2 contains a N-terminal collagenous 7S domain (aa 26-183), a central major collagenous domain that has over 20 interruptions of repeated -Gly-Xaa-Yaa- sequences, and the C-terminal non-collagenous (NC1) domain (aa 1489-1712). Each type of collagen IV is a heterotrimer composed of three chains. Formation of these heterotrimers is initiated by the NC1 domain interactions and is accompanied by folding of the collagenous domains into triple helices. Type IV collagens also contain numerous cysteine residues that are involved in inter- and intramolecular disulfide bonding. Mutations in COL4A2 gene are known to cause brain small vessel disease that can lead to intracranial hemorrhage, fluid-filled cysts within the cerebral hemisphere, and delayed psychomotor development. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Khoshnoodi, J., et al. (2008). Microsc. Res. Tech. 2008 May; 71(5): 357-370).

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal half of human collagen Type IV 2.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany