跳转至内容
Merck
CN
登录查看公司和协议定价

About This Item

UNSPSC代码:
12352203
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

生物来源

rabbit

质量水平

重组

expressed in HEK 293 cells

偶联物

unconjugated

抗体形式

purified antibody

抗体产品类型

primary antibodies

克隆

1B5, recombinant monoclonal

描述

recombinant, expressed in HEK 293 cells

产品线

ZooMAb® learn more

表单

lyophilized

分子量

calculated mol wt 57.95 kDa
observed mol wt ~65.00 kDa

纯化方式

using Protein A

种属反应性

human

包装

antibody small pack of 25

环保替代产品特性

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

增强验证

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

技术

affinity binding assay: suitable
immunohistochemistry: suitable
western blot: suitable

同位素/亚型

IgG

表位序列

C-terminal half

Protein ID登记号

UniProt登记号

环保替代产品分类

运输

ambient

储存温度

2-8°C

基因信息

human ... ALPP(250)

相关类别

一般描述

Alkaline phosphatase, placental type (UniProt: P05187; also known as EC:3.1.3.1, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1) is encoded by the ALPP (also known as PLAP) gene (Gene ID: 250) in human. Placental alkaline phosphatase (ALPP) is a metalloenzyme belonging to the alkaline phosphatase family. It is characterized by its ability to hydrolyze phosphate monoesters, a function essential for various biological processes including dephosphorylation reactions. ALPP forms a homodimer, each monomer containing a catalytic site that binds one magnesium and two zinc ions. It is anchored in the plasma membrane by a phosphatidylinositol-glycan moiety (PI-glycan) that is added to the nascent peptide chain after the removal of 29 amino acids from the COOH-terminus. ALPP is predominantly expressed in placental trophoblasts. It is upregulated in alcohol-related liver disease and in certain cancers, such as ovarian, colon, non-small lung cancer and testicular germ cell tumors. ALPP is used as a prognostic marker in pancreatic adenocarcinoma and a target for cancer immunotherapy. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Llinas, P., et al., (2005), J Mol Biol, 350(3), 441-451; Lowe, M. E., (1992), J Cell Biol, 116 (3), 799 807)
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

特异性

Clone 1B5 is a ZooMAb® rabbit recombinant monoclonal antibody that detects ALPP. It targets an epitope within 16 amino acids from the C-terminal half.

免疫原

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of human ALPP.

应用

Quality Control Testing

Evaluated by Western Blotting in human placenta tissue lysate.




Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ALPP in human placenta tissue lysate.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected ALPP in human placenta tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound with ALPP peptide with at least one thousand-fold (1,000X) higher affinity than with non-specific control peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

制备说明

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

未找到合适的产品?  

试试我们的产品选型工具.


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

没有发现合适的版本?

如果您需要特殊版本,可通过批号或批次号查找具体证书。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持