581402-U
Ascentis® C8 (3 µm) HPLC Columns
L × I.D. 15 cm × 2.1 mm, HPLC Column
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关于此项目
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
L × i.d.:
15 cm × 2.1 mm
Particle size:
3 μm
Matrix active group:
C8 (octyl) phase
Pore size:
100 Å
Matrix:
fully porous particle, silica gel high purity, spherical
产品名称
Ascentis® C8 HPLC 色谱柱, 3 μm particle size, L × I.D. 15 cm × 2.1 mm
material
stainless steel column
agency
suitable for USP L7
product line
Ascentis®
feature
endcapped
manufacturer/tradename
Ascentis®
packaging
1 ea of
extent of labeling
15% Carbon loading
parameter
≤70 °C temp. range, 400 bar pressure (5801 psi)
technique(s)
HPLC: suitable, LC/MS: suitable
L × I.D.
15 cm × 2.1 mm
surface area
450 m2/g
surface coverage
4.0 μmol/m2
impurities
<5 ppm metals
matrix
fully porous particle, silica gel high purity, spherical
matrix active group
C8 (octyl) phase
particle size
3 μm
pore size
100 Å
operating pH range
2-8
application(s)
food and beverages
separation technique
reversed phase
Quality Level
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General description
Ascentis 系列色谱柱是 Supelco 科学家推出的第四代 HPLC 色谱柱技术。Ascentis 系列色谱柱是以高纯硅胶为基质，孔径 100Å，硅胶粒径为 3、5 和 10 微米。该色谱柱用于小分子的应用，柱内径可从微径柱（1.0mm 内径）扩展到制备尺寸（50mm 内径）。该系列包括 C18、C8、苯基、Si 和内嵌极性基团相（反相酰胺）。
Ascentis C8 色谱柱重现性好，对极性化合物具有优良的选择性。该键合相在高含水量的流动相和高有机相含量的流动相中均有优良的性能。
Ascentis C8 色谱柱重现性好，对极性化合物具有优良的选择性。该键合相在高含水量的流动相和高有机相含量的流动相中均有优良的性能。
Features and Benefits
- 对疏水性分子的保留性极好
- 峰形极佳
- LC-MS 分离流失低
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Ebenezer B Asafu-Adjaye et al.
Journal of pharmaceutical and biomedical analysis, 43(5), 1854-1859 (2007-02-16)
Gastrointestinal stability of venlafaxine was evaluated in vitro in simulated gastric (SGF) and intestinal (SIF) fluids using a stability indicating HPLC method. The method was validated using a 5 microm Ascentis C18 column (150 mm x 4.6 mm) and mobile
Mahmoud Hasan et al.
Journal of pharmaceutical and biomedical analysis, 100, 341-347 (2014-09-10)
Clodronate belongs to the class of bisphosphonates which are used for the treatment of bone disorders. Due to its high polarity it has a low and highly variable oral bioavailability which results in low plasma concentrations and requires sensitive bioanalytical
Luana Magalhães et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1008, 98-107 (2015-12-08)
Nucleoside triphosphate diphosphohydrolase (NTPDase) is an enzyme belonging to the apyrase family that participates in the hydrolysis of the nucleosides di- and triphosphate to the corresponding nucleoside monophosphate. This enzyme underlies the virulence of parasites such as Leishmania. Recently, an
Sreenivasa Rao Chitturi et al.
Journal of pharmaceutical and biomedical analysis, 55(1), 31-47 (2011-02-15)
This paper proposes a simple and selective RP-HPLC method for the determination of process impurities and degradation products (degradants) of atazanavir sulfate (ATV) drug substance. Chromatographic separation was achieved on Ascentis(®) Express C8, (150mm×4.6mm, 2.7μm) column thermostated at 30°C under
Luigi Silvestro et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 878(30), 3134-3142 (2010-10-20)
Quantitative methods using LC-MS/MS allow achievement of adequate sensitivity for pharmacokinetic studies with clopidogrel; three such methods, with LLOQs as low as 5 pg/mL, were developed and fully validated according to the well established FDA 2001 guidelines. The chromatographic separations
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