Primary human Hepatocytes are cells directly harvested from whole human livers and are used by researchers in vitro to model the physiology and function of hepatocytes. As one of the major cell types of the liver, Hepatocytes function in protein, carbohydrate, and lipid metabolism and help to maintain liver homeostasis1. Because these primary cells can be cryopreserved and then cultured in vitro, they are crucial tools for studying enzyme induction, toxicity, drug screening, transporter efflux activity, potential drug-drug interactions, and disease modeling.
Our collection of cryopreserved human plateable Hepatocytes comprises a homogeneous cell population and is available in diverse pack sizes, including a validated product suitable for use in small volumes within 96-well plates. Each donor provides documented consent for research use of non-transplantable organs or tissues. Every cell lot undergoes extensive characterization, including induction data, enzyme profiling data, and morphological and confluency assessments, with a guaranteed post-thaw viability of ≥70%. This protocol outlines the steps for thawing, plating, and cultivating human plateable Hepatocytes.
Note: For Dexamethasone, dissolve 25 milligram into 32 milliLiter of ethanol (100%) to make 2 mM stock. For Linoleic acid, dissolve 100 mg into 2 mL of ethanol (100%) to make 50 mg/mL stock.
All protocols are performed within a Class II laminar flow biohood and with an aspirator unless otherwise specified. Incubators are humidified and are set to 37°C and 5% CO2. PPE should be worn such as gloves, lab coat, and safety glasses.
Plateable Hepatocytes were plated in HPM overnight on collagen coated plates. Media was changed to hepatocyte maintenance medium before the images were taken on the Millicell® DCI Digital Cell Imager. The plateable Hepatocytes show characteristic cobble stone morphology with well-defined borders.
Figure 1A.Primary plateable Hepatocytes imaged after overnight incubation. Imaged with Millicell® DCI Digital Cell Imager at 10x.
Figure 1B.Primary plateable Hepatocytes imaged after overnight incubation. Imaged at 20x magnification setting.
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