Discovery® HPLC Columns for Fast LC-MS Analysis
LC-MS Columns
The Discovery LC-MS line offers short length columns for fast LC-MS analysis that are rugged and dependable. Supelco offers a robust product that withstands column shock due to fast gradient turnaround times. Columns installed on LC-MS instruments are typically operated using gradients of 5%-95% organic with gradient cycle times of 2-3 minutes. Our column lifetime is maximized through the use of stable bonding chemistry, high purity silica and optimal proprietary packing procedures.
The Discovery line of HPLC columns offers excellent retentivity, selectivity and reproducibility for optimal results. LC-MS formats are now available with Discovery C18, C8, Cyano and HS F5 phases.
Discovery LC-MS Columns Deliver:
High Speed Separations
Discovery LC-MS columns can shorten analysis times to less than 4 minutes for the study of antibiotics.
Figure 1. Rifampicin on Discovery C18
| Column 1: | Discovery C18, 15 cm x 2.1 mm ID, 5 µM particles |
| Cat. No.: | 50495521 |
| Column 2: | Discovery C18, 2 cm x 2.1 mm ID, 5 µM particles |
| Cat. No.: | 577507-U |
| Mobile Phase: | 60:40, 33 mM HCO2H:CH3CN |
| Flow Rate: | 0.2 mL/min |
| Temp.: | 35 ºC |
| Det.: | ESI(+), full scan |
| Inj.1: | 6 µL |
| Inj.2: | 0.8 µL |
| Sample: | 10 mg/L in 50% CH3OH |
| Figure 2. Application of Short, Fast LC-MS Columns for Analysis of Veterinary Antibiotics on Discovery C18 and C8 Columns | ||||||||||||||||||||||||||||||||||||||||||
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| Discovery C18, A:B:C = 20:5:75 | Discovery C8, A:B:C = 20:10:70 |
| Column 1: | Discovery C18, 2 cm x 2.1 mm ID, 5 µM particles |
| Cat. No.: | 577507-U |
| Column 2: | Discovery C8, 2 cm x 2.1 mm ID, 5 µM particles |
| Cat. No.: | 577501-U |
| Column 3: | Discovery Cyano, 3 cm x 2.1 mm ID, 5 µM particles |
| Cat. No.: | 577514-U |
| Mobile Phase: | A=50 mM HCO2H, B=water, C=90:10 CH3OH:CH3CN |
| Flow Rate: | 0.2 mL/min |
| Temp.: | 35 ºC |
| Inj.: | 1 µL |
| Det.: | ESI(+) |
| Sample: | |
| 1. | Erythromycin c (1 µg/mL) |
| 2. | Clarithromycin (1 µg/mL) |
Discovery C18
A:B:C = 20:35:45
Discovery C8
A:B:C = 20:35:45
Discovery Cyano
A:B:C = 20:40:40
Mechanical Stability via Fast Gradient Testing
The Supelco Discovery LC-MS HPLC columns are rigorously tested in order to provide the highest mechanical stability under repeated gradient cycles. Table 1 shows of the impact on column efficiency, peak shape, retention times and back pressure after 1000 cycles of a 2-minute rapid (ballistic) gradient with 1-minute equilibration period.
There is no loss of column efficiency or increase in tailing or column backpressure, and no change in retention time. See Table 2 for details of testing.
Discovery C18 Bleed Analysis
Mass spectral responses from background ions often inhibit both qualitative and quantitative analysis in LC-MS experiments. Common sources of background ions include the solvents, interface or system contamination, and HPLC column bleed. The presence of background ions can be reduced by using high quality solvents such as LC-MS, maintaining a clean LC-MS system, and by using high quality HPLC stationary phases from Supelco.
Supelco utilizes technologically advanced bonding chemistry and highly purified silica to minimize the potential for column bleed.
LC-MS Bleed Analysis
The Total Ion Chromatogram (TIC) shown in the top of Figure 4 shows the result from an MS detector of one gradient cycle with a Discovery C18, 2 cm x 2.0 mm ID column in-line. The two selective ion chromatograms (SIC) in the bottom of Figure 4 show the mass spectrum taken from the shaded region of the TIC (at the high organic content point in the gradient). The bottom right SIC shows that the Discovery C18 column does not introduce any additional masses that were not in the blank run (bottom left SIC). The Discovery C18 column exhibits low bleed for LC-MS analyses.
Total Ion Chromatogram
Discovery C18, 2 cm x 2.0 mm ID, acetonitrile gradient in presence of 0.1% formic acid / 5 cycles / ESI (+) mode acquisition
Figure 4.LC-MS Bleed Study.
Blank Run (no LC column) (19-21 min.)
Discovery C18 column (19-21 min.)
Ordering Information
Related Literature
- Application Report 46 - Drugs of Abuse on Discovery HS F5 (T003046)
- Application Report 134 - Separation of Dopamine and Related Compounds using Discovery HS F5 with Mass Spectrometric Detection (T004134)
- Application Report 136 - Resolution of Bilirubin and Biliverdin on Discovery C18 (T004136) [Note: With MS compatible mobile phase.]
- 2004 Poster Presentation - Advantages of Polar, Reversed-Phase HPLC Columns for the Analysis of Drug Metabolites (T404045)
How to Contact Supelco
In USA:
Phone: 800-247-6628 or 814-359-3441
fax: 800-325-5052
email: techservice@sial.com
Outside the USA: click here
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