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CS0790

Sigma-Aldrich

Antioxidant Assay Kit

sufficient for 200 tests

Synonym(s):

Oxidative Stress Detection Kit

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CN¥7,443.86

CN¥7,443.86


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1 KIT
CN¥7,443.86

About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

CN¥7,443.86


Please contact Customer Service for Availability

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usage

sufficient for 200 tests

Quality Level

detection method

colorimetric

shipped in

wet ice

storage temp.

2-8°C

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This Item
GRSAMAK334MAK187
Quality Level

200

Quality Level

200

Quality Level

-

Quality Level

-

usage

sufficient for 200 tests

usage

sufficient for 100 tests

usage

sufficient for 100 colorimetric tests

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

General description

Free radicals or reactive oxygen species (ROS) are produced during biochemical redox reactions as part of normal physiological cell metabolism (protection from infectious organisms) and as a response to environmental factors such as UV light, cigarette smoke, environmental pollutants, and g-radiation. Once formed, ROS attack cellular components causing damage to lipids, proteins, and DNA, which can initiate numerous diseases, including cancer, atherosclerosis, rheumatoid arthritis, diabetes, liver damage, and central nervous system disorders.
In the presence of antioxidants the radical cation is suppressed to an extent dependent on the activity of the antioxidant and the color intensity is decreased proportionally. Trolox, a water-soluble vitamin E analogue, serves as a standard or a control antioxidant.

Application

Antioxidant assay kit provides all of the reagents required for an efficient measurement of the total antioxidant capacity of plasma, serum, urine, saliva, cells, and tissue lysates.[1] It has also been used to measure the radical cation (2,2′-azino-di-[3-ethylbenzthiazoline sulphonate]) (ABTS+) scavenging capacity.[2][3]
It was tested on A431 and CHO cell lysates; rat brain, liver, and kidney lysates; human plasma, serum, urine, and saliva.

Suitability

Suitable for the measurement of the total antioxidant capacity of plasma, serum, urine, saliva, cells, and tissue lysates

Principle

The principle of the antioxidant assay is formation of a ferryl myoglobin radical from metmyoglobin and hydrogen peroxide, which oxidizes the ABTS (2,2¢-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) to produce a radical cation, ABTS·+ , a soluble chromogen that is green in color and can be determined spectrophotometrically at 405 nm.

Preparation Note

Reagents are to be prepared with ultrapure water.

Reconstitution

Myoglobin Solutions are to be stored at -20C after reconstitution.
Trolox Working Solution is to be stored at -20C after reconstitution.
ABTS Substrate Solution is to be stored at 4C for 2 weeks or at -20C for 1 month.

Legal Information

Kit Components Only

Product No.
Description

  • Assay Buffer 10X 30 mL

  • Stop Solution 20 mL

  • Hydrogen Peroxide, 3% solution 1 mL

  • Myoglobin from horse heart 2 x 1

  • (±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox®) 2 x 1

  • ABTS, 10 mg tablets 4 tab

  • Phosphate-Citrate Buffer, pH 5 4 tab

related product

Product No.
Description
Pricing

Pictograms

CorrosionExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

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  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the pH of kit component A3605 in the Antioxidant Assay Kit, product CS0790?

    The pH of kit component A3605 in the Antioxidant Assay Kit, product CS0790, is approximately 5,  at 25°C. The adequacy/suitability of the pH of the assay buffer is assessed via the assay itself, and is tested for each lot of kits.

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