usage
sufficient for 200 tests
Quality Level
detection method
colorimetric
shipped in
wet ice
storage temp.
2-8°C
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General description
在存在抗氧化剂的条件下，自由基阳离子受抑制的程度取决于抗氧化剂活性，且颜色深度成比例下降。Trolox是一种水溶性维生素E类似物，可作为标准抗氧化剂或对照抗氧化剂。
自由基或活性氧(ROS)在生化氧化还原反应过程产生，是正常生理细胞新陈代谢（免受传染性生物体侵害）的参与成分，并可对紫外线、香烟烟雾、环境污染物和伽马辐射等环境因素作出响应。ROS形成之后即会攻击细胞成分，破坏脂质、蛋白质和DNA，从而引发多种疾病，包括癌症、动脉粥样硬化、类风湿性关节炎、糖尿病、肝损伤和中枢神经系统疾病。
Application
抗氧化测定试剂盒提供了有效测定血浆、血清、尿液、唾液、细胞和组织裂解液的总抗氧化能力所需的所有试剂。它也被用来测定自由基阳离子（2,2′-连氮基-二-[3-乙基苯并噻唑啉磺酸盐]）（ABTS +）的清除能力。
它已在A431和CHO细胞裂解物，大鼠脑、肝和肾裂解物，人血浆、血清、尿液和唾液中进行了测试。
适于测定血浆、血清、尿液、唾液、细胞和组织裂解液的总抗氧化能力
Biochem/physiol Actions
抗氧化剂测定的原理是：由肌红蛋白和过氧化氢形成亚铁肌红蛋白自由基，该自由基将ABTS（2,2¢-联氮双(3-乙基苯并噻唑啉-6-磺酸）氧化生成自由基阳离子ABTS·+（绿色可溶性色原），从而在405 nm处用分光光度法测定。
Preparation Note
肌红蛋白溶液保存在-20℃条件下。
Trolox工作溶液保存在-20℃条件下。
ABTS底物溶液可在4℃下保存两周或-20℃下保存1个月。
Trolox工作溶液保存在-20℃条件下。
ABTS底物溶液可在4℃下保存两周或-20℃下保存1个月。
试剂用超纯水配置。
Legal Information
仅试剂盒组分
产品编号
说明
- Assay Buffer 10X 30 mL
- Stop Solution 20 mL
- Hydrogen Peroxide, 3% solution 1 mL
- Myoglobin from horse heart 2 x 1
- (±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox®) 2 x 1
- ABTS, 10 mg tablets 4 tab
- Phosphate-Citrate Buffer, pH 5 4 tab
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
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