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Merck
CN

17-10086

Sigma-Aldrich

EZ-Magna ChIP® A/G Chromatin Immunoprecipitation Kit

Single day chromatin immunoprecipitation (ChIP) kit containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using magnetic A/G beads. Control primers included.

Synonym(s):

Magnetic ChIP Kit, Magnetic Chromatin Immunoprecipitation

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.52

Key Documents

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Quality Level

100

manufacturer/tradename

Magna ChIP®

technique(s)

immunoprecipitation (IP): suitable

shipped in

dry ice

Related Categories

General description

Chromatin Immunoprecipitation (ChIP) is a powerful technique for mapping the in vivo distribution of proteins associated with chromosomal DNA. These proteins can be histone subunits and post-translational modifications or other chromatin associated proteins such as transcription factors, chromatin regulators, etc. Additionally, ChIP can be used to identify regions of the genome associated with these proteins, or conversely, to identify proteins associated with a particular region of the genome. ChIP methodology often involves protein-DNA and protein-protein cross-linking, fragmentation of the cross-linked chromatin, and subsequent immunoprecipitation of chromatin with an antibody specific to a target protein. The DNA fragments isolated in complex with the target protein can be identified by a variety of methods including PCR, DNA microarray and DNA sequencing. Standard or quantitative PCR can be performed to verify whether a particular DNA sequence (the gene or region of the genome) is associated with the protein of interest. The combination of ChIP and promoter or genomic tiling microarrays (ChIP-chip) allows genome-wide identification of DNA-binding sites for chromatin-associated proteins with precise resolution. Alternatively, high-throughput sequencing of libraries constructed from immunoprecipitated chromosomal DNA (ChIP-Seq) is a powerful alternative to ChIP-chip in mapping the protein-DNA interactions across mammalian genomes.
Unlike standard ChIP protocols that can be laborious and time consuming, the Magna ChIP kit protocol can reduce the amount of time required to perform a ChIP experiment from three days to one. Additionally, the smaller Magna ChIP reaction volume increases the relative concentration of the antibody enabling the ChIP reaction to be performed with reduced amounts of both antibody and sheared chromatin. Finally because this kit uses a blend of protein A and protein G beads, a wider range of antibody isotypes can be used than A or G alone. This allows a wider variety of antibodies to be used and avoids the need to purchase separate kits for protein A and protein G based immunoprecipitation. Because Magna ChIP kits use paramagnetic beads they are compatible with automated high throughput platforms, thus allowing a large number of ChIP reactions to be carried out simultaneously. Features & Benefits:
  • Magnetic bead-based rapid protocol allows performance of ChIP in as little as 1 day
  • Blend of protein A+G bead blend allows ChIP using a broader range of antibodies than A or G alone
  • Negative and positive control antibodies and control primer set to simplify validation of experimental procedure
  • Includes spin columns to make DNA purification easier and more reliable - no more messy phenol-chloroform extractions
  • Complete kit with all required reagents for reliable and reproducible results
  • Compatible with ChIPAb+ validated antibody and primer sets

Chromatin Immunoprecipitation (ChIP) is an important technique allowing the analysis of in vivo interactions of proteins with genomic DNA. Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists. One can measure different proteins localized to a specific region of the genome, or the genome wide distribution of a specific protein. Another powerful application of this technique is to analyze changes in histone modifications that correlate with processes like transcription, mitosis or DNA repair.

Application

Research Category
Epigenetics & Nuclear Function
Used to detect/quantify: Protein A+G

Packaging

Kit capacity: 22 chromatin immunoprecipitation assays

Physical form

Two boxes containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions. Supplied buffers are sufficient to generate chromatin from up to five 15 cm plates of cultured cells, each plate providing up to 10 chromatin preparations (varies with cell and assay type).

Preparation Note

Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 1 year from date of shipment when stored as directed.

Other Notes

Magnetic Protein A/G Beads

ChIP Dilution Buffer

Low Salt Wash Buffer

High Salt Wash Buffer

LiCl Wash Buffer

TE Buffer

Cell Lysis Buffer

Nuclear Lysis Buffer

ChIP Elution Buffer (w/o Proteinase K)

Proteinase K

RNase A

10X Glycine

10X PBS

Protease Inhibitor Cocktail II

Anti-RNA polymerase II, clone CTD4H8

Normal Mouse IgG

Control Primers

Spin Filters

Collection Tubes

Bind Reagent A

Wash Reagent B

Elution Reagent C

Legal Information

MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2

Storage Class Code

3 - Flammable liquids

Flash Point(F)

55.4 °F

Flash Point(C)

13 °C

Regulatory Information

新产品
This item has

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linc-UBC1 physically associates with polycomb repressive complex 2 (PRC2) and acts as a negative prognostic factor for lymph node metastasis and survival in bladder cancer.
He, W; Cai, Q; Sun, F; Zhong, G; Wang, P; Liu, H; Luo, J; Yu, H; Huang, J; Lin, T
Biochimica et Biophysica Acta null
Long Noncoding RNA lncCAMTA1 Promotes Proliferation and Cancer Stem Cell-Like Properties of Liver Cancer by Inhibiting CAMTA1.
Ding, LJ; Li, Y; Wang, SD; Wang, XS; Fang, F; Wang, WY; Lv, P; Zhao, DH; Wei, F; Qi, L
International Journal of Molecular Sciences null
Endogenous miRNA sponge lincRNA-RoR regulates Oct4, Nanog, and Sox2 in human embryonic stem cell self-renewal.
Wang, Y; Xu, Z; Jiang, J; Xu, C; Kang, J; Xiao, L; Wu, M; Xiong, J; Guo, X; Liu, H
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LIFR?-CT3 induces differentiation of a human acute myelogenous leukemia cell line HL-60 by suppressing miR-155 expression through the JAK/STAT pathway.
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Getting started with ChIP-seq: experimental design to data analysis

Chromatin-immunoprecipitation (ChIP) followed by next generation sequencing (ChIP-seq) of the immunoprecipitated DNA is a powerful tool for the investigation of protein:DNA interactions. To perform ChIP-seq, chromatin is isolated from cells or tissues (with or without chemical crosslinking) and fragmented. Antibodies recognizing chromatinassociated proteins of interest are used to enrich the sample for specific chromatin fragments. The DNA is recovered, sequenced on various NGS platforms, and aligned to a reference genome to determine specific protein binding loci. ChIP-seq studies have increased our knowledge of transcription factor biology, DNA methylation and histone modifications.

Magna ChIP™ protein A/G bead blends simplify the ChIP process and reduce background signals

Chromatin immunoprecipitation (ChIP) has been widely adapted for the study of gene-specific and genome-wide distribution of specific DNA- and RNA-binding proteins or protein modifications. Similar to standard protein immunoprecipitation assays, ChIP involves isolation of immunocomplexes using a solid medium, such as agarose or magnetic beads, coupled to either IgG binding recombinant protein A or protein G. In a typical ChIP experiment either protein A or G is selected for enrichment depending on the antibody isotype. However, proteins A and G possess differing affinities for human and mouse IgGs. Complicating this choice, for some antibody isotypes there is affinity for both protein A and G. In addition, we have observed that independent of the isotype the affinity of a specific antibody for protein A or G can vary depending on the specific clone, purification method, and source.

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