MAB318

Anti-Tyrosine Hydroxylase Antibody

Name: Anti-Tyrosine Hydroxylase Antibody
Brand: MM

CHEMICON^®, mouse monoclonal, LNC1

别名:

TH, Tyrosine Monooxygenase, Tyrosine 3-monooxygenase

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关于此项目

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

主要文件

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产品名称

抗酪氨酸羟化酶抗体,克隆LNC1, ascites fluid, clone LNC1, Chemicon^®

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

LNC1, monoclonal

species reactivity

zebrafish, mouse, rat, vole, human, frog, lizard, monkey, chicken

manufacturer/tradename

Chemicon^®

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NM_000360.3

UniProt accession no.

P07101

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... TH(7054)
rat ... Th(25085)

Biochem/physiol Actions

可识别调节性N末端外部的表位可通过Western blot识别一个约59-61 kDa的蛋白。在Western blot检测时不要与以下酶发生反应：多巴胺-β-羟化酶、苯丙氨酸羟化酶、色氨酸羟化酶、脱氢蝶呤还原酶、Sepaapterin还原酶或苯乙醇胺-N-甲基转移酶（PNMT）。

Application

免疫组化（石蜡）：
之前一个批次的1:200-1:400稀释液被用于IH。4% PFA固定的冷冻切片；4% PFA固定的石蜡切片1:100（Barrachina, M. et al., 2003）。针对石蜡切片，Barrachina曾报道过采用柠檬酸微波微波抗原回收成功进行过染色，但其他方法也应该可以使用。

免疫沉淀：
之前一个批次的该抗体被用于IP（Perez, 2002）。

终端用户需要对最佳的工作稀释比和实验方法进行确定。

使用该抗-酪氨酸氢化酶抗体进行酪氨酸氢化酶检测，克隆LNC1已经验证可用于IH、IH(P)、IP &WB并有超过85篇的产品引文。

研究子类别
神经递质 & 受体

神经元 & 胶质标记

研究类别
神经科学

Analysis Note

对照
人脑组织，提取自用Forskolin处理（40 nM，30 min）的3T3细胞。

通过Western Blot对小鼠大脑裂解液进行常规评估。

Western blot：
该批次1:1000的稀释液对10 μg 小鼠大脑裂解液中的酪氨酸羟化酶进行了检测。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

59-63kda

酪氨酸羟化酶在肾上腺素能神经元的生理中起重要作用。它是从酪氨酸生物合成儿茶酚胺多巴胺和去甲肾上腺素的第一种酶和限速酶。因此，TH是一种有用的多巴胺能和去甲肾上腺素能神经元标志物。TH酶活性维持需要亚铁离子作为辅因子，且人们普遍认为其受到磷酸化作用调控。目前至少鉴定出了四种因可变剪接而产生的人TH异构体。

Immunogen

从PC12细胞纯化的酪氨酸羟化酶

Other Notes

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

Physical form

含有3％BSA的腹水小鼠单克隆IgG1κ液体。不含防腐剂。

未纯化

Preparation Note

自收到之日起在-20ºC可稳定保存1年。

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Age and region-specific responses of microglia, but not astrocytes, suggest a role in selective vulnerability of dopamine neurons after 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine exposure in monkeys.

Kanaan, NM; Kordower, JH; Collier, TJ

Glia null

Cholinergic axons in the rat ventral tegmental area synapse preferentially onto mesoaccumbens dopamine neurons.

Natalia Omelchenko et al.

The Journal of comparative neurology, 494(6), 863-875 (2005-12-31)

Cholinergic afferents to the ventral tegmental area (VTA) contribute substantially to the regulation of motivated behaviors and the rewarding properties of nicotine. These actions are believed to involve connections with dopamine (DA) neurons projecting to the nucleus accumbens (NAc). However

Characterization of the dopamine defect in primary cultures of dopaminergic neurons from hypoxanthine phosphoribosyltransferase knockout mice.

D W Smith et al.

Molecular therapy : the journal of the American Society of Gene Therapy, 1(5 Pt 1), 486-491 (2000-08-10)

Lesch-Nyhan disease (LND) is an X-linked metabolic disorder caused by lack of activity of the purine salvage enzyme hypoxanthine phosphoribosyltransferase (HPRT) and characterized by hyperuricemia and debilitating neurological manifestations. The mechanisms underlying the neuropathology are not well understood and the

Striatal overexpression of DeltaFosB reproduces chronic levodopa-induced involuntary movements.

Cao, X; Yasuda, T; Uthayathas, S; Watts, RL; Mouradian, MM; Mochizuki, H; Papa, SM

The Journal of Neuroscience null

Pre-Bötzinger complex receives glutamatergic innervation from galaninergic and other retrotrapezoid nucleus neurons.

Genrieta Bochorishvili et al.

The Journal of comparative neurology, 520(5), 1047-1061 (2011-09-22)

The retrotrapezoid nucleus (RTN) contains CO(2) -responsive neurons that regulate breathing frequency and amplitude. These neurons (RTN-Phox2b neurons) contain the transcription factor Phox2b, vesicular glutamate transporter 2 (VGLUT2) mRNA, and a subset contains preprogalanin mRNA. We wished to determine whether

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Anti-Tyrosine Hydroxylase Antibody

CHEMICON®, mouse monoclonal, LNC1

选择尺寸

关于此项目

主要文件

属性

产品名称

biological source

antibody form

antibody product type

clone

species reactivity

manufacturer/tradename

technique(s)

isotype

NCBI accession no.

UniProt accession no.

shipped in

target post-translational modification

Quality Level

Gene Information

相关类别

说明

Biochem/physiol Actions

Application

Analysis Note

Disclaimer

General description

Immunogen

Other Notes

Physical form

Preparation Note

Legal Information

安全信息

存储类别

wgk

flash_point_f

flash_point_c

文件

分析证书(COA)

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CHEMICON^®, mouse monoclonal, LNC1