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Merck
CN

MAB4304

抗-阶段特异性胚胎抗原-4抗体，克隆MC-813-70

clone MC-813-70, Chemicon®, from mouse

别名:

SSEA-4

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
MC-813-70, monoclonal
Species reactivity:
human, mouse
Application:
ELISA, FACS, IF, IHC
Citations:
216

主要文件

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biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MC-813-70, monoclonal

species reactivity

human, mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, flow cytometry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable

input

sample type: mouse embryonic stem cell(s)
sample type: human embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

isotype

IgG3

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

相关类别

General description

通常用于鉴定未分化的人类胚胎干细胞（hESC）的细胞表面标志物最初被表征为mESC、小鼠胚胎性癌(EC)或人类EC细胞的标志物。阶段特异性胚胎抗原3（SSEA-3）和SSEA-4被广泛用作定义人和小鼠ESC的细胞表面标志物。SSEA-3和SSEA-4在未分化的hESC上表达，但在未分化的mESC上不表达。

Immunogen

人胚胎性癌细胞系2102Ep

Application

免疫组织化学： 该抗体的一个先前批次已被用于IH。

免疫荧光：该抗体的一个先前批次已被用于IF。

ELISA： 该抗体的一个先前批次已被用于ELISA。

流式细胞术: 建议起始范围为10-20 µg/mL。

最佳工作稀释度必须由最终用户进行确定。
抗阶段特异性胚胎抗原-4抗体（克隆MC-813-70）是一种抗阶段特异性胚胎抗原-4的抗体，用于FC、ELISA、IF、IH。
研究子类别
多能性&早期分化
研究类别
干细胞研究

Biochem/physiol Actions

该抗体与人四癌干细胞(EC)、人胚胎生殖细胞(EG)和人胚胎干细胞(ES)表面表达的阶段特异性胚胎抗原-4（SSEA-4）反应。 未分化的鼠EC、ES和EG细胞没有明显的免疫反应性。 人EC细胞分化后，SSEA-4的表达下调。 相反，鼠EC和ES细胞的分化可能伴随着SSEA-4表达的增加。

Physical form

形式：纯化
纯化的小鼠单克隆IgG3液体，溶于含0.02 M PBS、0.25 M NaCl（pH 7.6）和0.1%叠氮化钠的缓冲液中
纯化蛋白A

Preparation Note

自接收之日起1年内保持稳定。

Analysis Note

对照
多能人胚胎干(ES)细胞

Other Notes

浓度：关于批次特定浓度请参见检验报告。

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

除非我们的目录或产品随附的其他公司文件中另有说明，否则我们的产品预期仅用于研究用途，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人类或动物的任何类型的消费或应用。

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存储类别

10 - Combustible liquids

wgk

WGK 2

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James A Byrne et al.
PloS one, 4(9), e7118-e7118 (2009-09-24)
The derivation of induced pluripotent stem cells (iPSCs) provides new possibilities for basic research and novel cell-based therapies. Limitations, however, include our current lack of understanding regarding the underlying mechanisms and the inefficiency of reprogramming. Here, we report identification and
Hidehito Saito et al.
Stem cells international, 2016, 8394960-8394960 (2016-04-09)
Induced pluripotent stem cells (iPSCs) derived from somatic cells of patients hold great promise for autologous cell therapies. One of the possible applications of iPSCs is to use them as a cell source for producing autologous lymphocytes for cell-based therapy
Lei Kang et al.
Stem cells and development, 25(7), 556-568 (2016-02-18)
Podocalyxin-like protein (PODXL) is a member of CD34 family proteins. It is the protein that carries many post-translational epitopes responsible for various pluripotent surface markers including TRA-1-60, TRA-1-81, GCTM2, GP200, and mAb84. However, PODXL has not attracted the attention of
Akihito Hashimoto et al.
International heart journal, 57(1), 112-117 (2015-12-18)
Duchenne muscular dystrophy (DMD) is caused by mutations in the DMD gene which encodes dystrophin protein. Dystrophin defect affects cardiac muscle as well as skeletal muscle. Cardiac dysfunction is observed in all patients with DMD over 18 years of age
Epigenetic characterization of the FMR1 gene and aberrant neurodevelopment in human induced pluripotent stem cell models of fragile X syndrome.
Sheridan, SD; Theriault, KM; Reis, SA; Zhou, F; Madison, JM; Daheron, L; Loring, JF; Haggarty, SJ
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