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Merck
CN

I3146

ITS液体培养基补充剂（100x）

liquid, sterile-filtered, BioReagent, suitable for cell culture

别名:

Human ITS Liquid Media, Insulin-transferrin-sodium selenite

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关于此项目

NACRES:
NA.75
UNSPSC Code:
12352205

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sterility

sterile-filtered

product line

BioReagent

form

liquid

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

impurities

endotoxin, tested

shipped in

ambient

storage temp.

2-8°C

Quality Level

500

相关类别

Application

胰岛素-转铁蛋白-硒添加剂（100×ITS液体培养基添加剂）作为培养基补充剂适用于：
  • α小鼠肝12细胞(AML-12)
  • 大鼠胎儿睾丸外植体
  • 人牙髓干细胞(DPSC)

Biochem/physiol Actions

  • 胰岛素：一种多肽激素，可促进葡萄糖和氨基酸的摄取，这种特性可能与观察到的有丝分裂作用有关。
  • 转铁蛋白：铁运输蛋白。
  • 硒：通常由血清提供的必需微量元素。

Preparation Note

使用不含酚红的Earle′平衡盐溶液（EBSS）制备。

Other Notes

ITS是人重组胰岛素、人转铁蛋白和亚硒酸钠的一种混合物。它是一种通用的细胞补充剂设计用于含有丙酮酸钠的非复合型培养基（如MEM、RPMI-1640）及复合型培养基（如Ham?s F-12、DME/F-12、MEM）。
含有1.0 mg/ml人重组胰岛素，0.55 mg/ml 人转铁蛋白（基本上不含铁）以及0.5 μg/ml 100x浓度的亚硒酸钠。

存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000425206
0000425206
0000416500
0000416500
0000366647

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细胞培养中的转铁蛋白

转铁蛋白和其他细胞培养成分如何影响无血清、无蛋白细胞培养系统（用于生物制造包括单克隆抗体在内的异源蛋白）的性能。本页介绍转铁蛋白的体外化学和生物化学。

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