G1163
O-糖苷酶 来源于肺炎链球菌
recombinant, expressed in E. coli, buffered aqueous solution
别名:
α-内切乙酰氨基半乳糖苷酶, O-糖酵素
重组
expressed in E. coli
质量水平
偶联物
(O-linked)
表单
buffered aqueous solution
分子量
180 kDa
浓度
≥800 units/mL
运输
wet ice
储存温度
2-8°C
生化/生理作用
从糖蛋白上释放未取代的Ser-和Thr-连接β-Gal-(1→3)-α-GalNAc（核心1型O-聚糖)。 用唾液酸、乳糖胺（半乳糖-N-乙酰氨基葡萄糖）或岩藻糖取代的二糖核心将阻止水解并防止寡糖从蛋白质中释放出来。在使用O-糖苷酶切割之前，可能需要使用糖酵解酶进行预处理以从O-聚糖中去除取代糖。
包装
提供5×反应缓冲液，250mM NaH2PO4，pH 5.0。
外形
溶于50 mM磷酸钾，pH 7.5
分析说明
筛查是否存在：β-半乳糖苷酶、α-甘露糖苷酶、β-己糖胺酶、α-岩藻糖苷酶、神经氨酸酶和蛋白酶。有关特定批次的信息，请参阅分析证书。
其他说明
在37 °C、pH 6.5下，一单位在每分钟内可水解1 μmole的对硝基苯基半乳糖N-生物苷（β-Gal-(1→3)-α-GalNAc-1→ΟC6H4NO2）。
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
K M Davis et al.
Protein expression and purification, 8(1), 57-67 (1996-08-01)
Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a 22-kDa, O-glycosylated protein. Because recombinant expression systems permitting a detailed analysis of the functional significance of HB-EGF glycosylation have not been described, a recombinant vaccinia virus designed to express HB-EGF was
A Shibuya
Pediatrics international : official journal of the Japan Pediatric Society, 43(6), 597-604 (2001-12-12)
Childhood hypoplastic anemia of unknown etiology had not existed until now. To assess pathophysiological differentiation in childhood hypoplastic anemia, we analyzed red cell membrane components in six children with hypoplastic anemia of unknown etiology. The six children all had chronic
Tongzhong Ju et al.
Glycobiology, 16(10), 947-958 (2006-06-10)
The common O-glycan core structure in animal glycoproteins is the core 1 disaccharide Galbeta1-3GalNAcalpha1-Ser/Thr, which is generated by the addition of Gal to GalNAcalpha1-Ser/Thr by core 1 UDP-alpha-galactose (UDP-Gal):GalNAcalpha1-Ser/Thr beta1,3-galactosyltransferase (core 1 beta3-Gal-T or T-synthase, EC2.4.1.122). Although O-glycans play important
G H Carpenter et al.
Oral microbiology and immunology, 14(5), 309-315 (1999-11-07)
Interactions between salivary glycoproteins and many oral bacteria have been shown to depend on O-linked glycans on salivary glycoproteins. Basic proline-rich proteins form the largest group of proteins within human parotid saliva. In the present study human parotid salivary glycoproteins
H Yamini Shrivastava et al.
Journal of biomolecular structure & dynamics, 21(5), 671-680 (2004-02-11)
In the present study, the impact of chromium(III) complexes ([Cr(salen)(H2O)2](+) (1), [Cr(en)3]3+ (2) and [Cr(EDTA)(H2O)]- (3)) on the biophysical properties of mucin like specific viscosity, zeta potential and particle size has been investigated. It is evident from the present investigation
商品
Learn about O-linked glycan strategies, O-glycosidase actions, how to remove sialic acid residues, β-Elimination, and O-glycan modifications.
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