LC-MS

Complete LC-MS Analysis
for Consistent Results

We provide a premier selection of proven analytical tools and consumables that meet the requirements of scientists who primarily use the liquid chromatography-mass spectrometry (LC-MS) technique for separation and analysis of drugs and biomolecules, or for analytical assays. Our selection of columns, solvents, standards and sample preparation products are exclusively designed for LC-MS, meeting the critical need for purity. You can also find LC-MS applications and method development advice, in addition to comprehensive overviews of our product offerings for both small and large molecule LC-MS workflows, below.

Workflow

Sample Collection

Sample Preparation

Standardization and Calibration

Chromatographic Separation & Analysis

Chromatographic and Separation Analysis - Featured Articles

Optimising the LC-MS Analysis of Biomolecules
We present an article concerning optimising the LC-MS analysis of biomolecules.
LC/MS Analysis of Nucleotides on SeQuant ZIC-cHILIC
HILIC separation is an alternative that permits sensitive MS detection and without the use of ion-pair reagents.
Quantification of Infliximab in Human Serum by LC-MS/MS Using A Full-Length Stable Isotope Labeled Internal Standard
Infliximab, a chimeric monoclonal antibody, is used to treat rheumatoid arthritis, psoriatic arthritis, Crohn’s disease, and other autoimmune diseases.
Multiplex Quantification of Infliximab and Adalimumab in Human Serum by LC-MS/MS Using Full-Length Stable Isotope Labeled Internal Standards
Infliximab and Adalimumab monoclonal antibodies (mAbs) are used widely to treat rheumatoid arthritis, psoriatic arthritis, and many autoimmune diseases by binding to tumor necrosis factor-alpha (TNFα) to reduce the inflammatory response.

Sample Collection and Preparation - Featured Articles

LC/MS/MS Method for Determination of Glyphosate, AMPA, and Glufosinate in Cereals
Glyphosate and related compounds are measured in oatmeal and infant cereal using ion-exchange polymer-based particles for HPLC and SPE. Low level detection was obtained.
Analysis of Active Cannabis Compounds (Cannabinoids) in Edible Food Products: Gummy Bears and Brownies
Rapid potency testing of marijuana-infused edibles using LC/MS on a biphenyl stationary phase detected eleven cannabinoids.

Related Product Resources

Application Note: Lopinavir Assay Method
Application Note: Workflow for High-Throughput Glycoprofiling of Rituximab
Brochure: BIOshell™ U/HPLC Columns with Fused-Core^® Technology
Brochure: A Practical Guide to HPLC
Brochure: HPLC and UHPLC Column Selection Guide
Flyer: Fentanyls
Poster: Tips & Tricks for Improved Quantitative and Qualitative LC-MS
Application Note: Workflow for Intact and Middle-Up Mass Analysis of Adalimumab
Brochure: Chiral HPLC Columns
Brochure: Supel™ BioSPME 96-Pin Devices
Application Note: Simplified LC-MS/MS Method for Glyphosate, AMPA and Glyfosinate in oat-based cereals

Application Note: LC-MS Analysis of PAHs in Grilled Meat
Application Note: Released N-linked Glycan Analysis Workflow of Adalimumab
Application Note: Peptide Mapping Analysis Workflow for Adalimumab
Application Note: LC-MS Analysis of 33 PFAS Compounds in 5 minutes – Ascentis^® Express PFAS
Application Note: LC-MS Analysis of PFAS Compounds in EPA Methods 537.1, 533 and 8327 – Ascentis^® Express PFAS
Flyer: Fast and High-Resolution LC-MS Separation with Ascentis^® Express PFAS HPLC Columns
Flyer: Per- and polyfluoroalkyl substances (PFAS)
Application Guide: Drugs of Abuse

Related Applications

Solid Phase Extraction (SPE)

Solid phase extraction (SPE) is a technique used for rapid, selective sample preparation and purification prior to chromatographic analysis (e.g. HPLC, GC, TLC). SPE is used to exchange sample matrices, concentrate analytes, and remove interferences and contaminants to improve data and protect the analytical column.

QuEChERS Sample Preparation Method

QuEChERS is a ‘Quick, Easy, Cheap, Effective, Rugged and Safe’ sample cleanup method used to prepare food and agricultural samples for pesticides analysis. QuEChERS is based on the Solid Phase Extraction (SPE) technique of sample preparation.

Sample Preparation by Filtration

Filtration separates substances based on physical or chemical properties. Lab samples are routinely filtered before analysis to reduce sample complexity and increase analyte purity. This allows scientists to generate higher quality analytical results.

Sample Purification

A range of purification methods may be used to prepare a sample prior to downstream analysis. Purification reduces sample complexity, removes interferences, and concentrates an analyte before analysis.

Small Molecule HPLC

Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).

Large Molecule HPLC

High performance liquid chromatography (HPLC) can be used to separate and identify different large biomolecules such as protein and peptides in a sample. It is based on the pumping of a sample with a solvent (mobile phase) through a column packed with sorbent material (stationary phase) at a high pressure.

Mass Spectrometry

Mass spectrometry (MS) is an analytical tool used to identify compounds, determine chemical structure, and assess isotopic abundance. In MS, samples are ionized, and the resulting ions are identified based on their mass-to-charge (m/z) ratios.

mRNA Development & Manufacturing

Our comprehensive portfolio of products and services provides everything you need for mRNA synthesis, purification, and formulation for development and manufacturing.