R7023
Pst I from Providencia stuartii
Restriction Enzyme
grade
Molecular Biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
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Application
PstI is a restiction enzyme used in molecular biology applications to cleave DNA at the recognition sequence 5′-CTGCA/G-3′, generating DNA fragments with 3′- cohesive termini.
Biochem/physiol Actions
Recognition sequence: 5′-CTGCA/G-3′
Cutting results: a 2-10-fold Pst I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 80 °C for 20 minutes.
Cutting results: a 2-10-fold Pst I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 80 °C for 20 minutes.
Physical form
Solution in 10 mM Tris-HCl, pH 7.0, 1 mM EDTA, 250 mM NaCl, 1 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.01% polydocanol (v/v) at 4 °C
Other Notes
Comment: Pst I requires optimal reaction conditions in order to avoid star activity.
Supplied with 10x Restriction Enzyme Buffer SH (B3657).
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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The mapping and sequence determination of the single site in phiX174am3 replicative form DNA cleaved by restriction endonuclease Pst I.
N L Brown et al.
FEBS letters, 65(3), 284-287 (1976-06-15)
T B Britschgi et al.
Applied and environmental microbiology, 57(6), 1707-1713 (1991-06-01)
The identification of the prokaryotic species which constitute marine bacterioplankton communities has been a long-standing problem in marine microbiology. To address this question, we used the polymerase chain reaction to construct and analyze a library of 51 small-subunit (16S) rRNA
Thomas Hiesberger et al.
The Journal of clinical investigation, 113(6), 814-825 (2004-04-07)
Hepatocyte nuclear factor-1beta (HNF-1beta) is a Pit-1, Oct-1/2, UNC-86 (POU)/homeodomain-containing transcription factor that regulates tissue-specific gene expression in the liver, kidney, and other organs. Humans with autosomal dominant mutations of HNF-1beta develop maturity-onset diabetes of the young type 5 (MODY5)
D I Smith et al.
Nucleic acids research, 3(2), 343-353 (1976-02-01)
We describe the isolation of a new class 2 restriction endonuclease from Providencia stuartii 164. Using the procedure of osmotic shock treatment, we have partially purified this enzyme (Pst 1) and have begun preliminary work to characterize its specificity and
E Müller et al.
Pflugers Archiv : European journal of physiology, 435(5), 705-712 (1998-04-16)
The influence of diuresis and antidiuresis on the expression of heat shock proteins (HSP) 25, 60, 72 and 73 in the renal cortex and outer and inner medulla of Wistar rats was analysed. Medullary osmolality was reduced by long-term diuresis
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