AAT Bioquest Helixyte™ Green ssDNA reagent

Helixyte™ Green ssDNA Quantifying Reagent is an excellent alternative for quantifying ssDNA and oligonucleotides with significantly improved sensitivity and selectivity. It is a positively charged fluorescent probe that binds to the hydrophobic pockets of ssDNA and forms a highly luminescent complex through the synergistic actions of stacking, hydrophobic forces, hydrogen bonding, and electrostatic interactions. Helixyte™ Green ssDNA reagent has extremely low inherent fluorescence that is significantly enhanced upon binding to ssDNA. The increased sensitivity of Helixyte™ Green ssDNA over absorbance at 260 nm enables researchers to quantify as little as 100 pg/mL oligonucleotide or ssDNA (~500 pg/mL) with a standard spectrofluorometer and fluorescein excitation and emission wavelengths.

Helixyte™ Green ssDNA reagent is manufactured by AAT Bioquest

Helixyte™ is a trademark of AAT Bioquest, SYBR® is the trademark of ThermoFisher