AAT Bioquest Azido-dT Phosphoramidite

Azido-dT Phosphoramidite is an excellent building block for introducing an azide group into an oligo via dT. Oligonucleotides bearing a single nucleosidic azide group can be readily modified with 2-5 equivalents of a tag-alkyne (e.g., a fluorescent dye alkyne). After the addition of complexed Cu(I), complete conversion to the labelled oligo can be achieved typically between 30 min and a few hours. After a simple precipitation step, labelled oligonucleotides can be recovered in high yields.

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