163783
Tricine
≥98%
Synonym(s):
N-[Tris(hydroxymethyl)methyl]glycine
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About This Item
Linear Formula:
(HOCH2)3CNHCH2CO2H
CAS Number:
Molecular Weight:
179.17
EC Number:
227-193-6
UNSPSC Code:
12352200
PubChem Substance ID:
Beilstein/REAXYS Number:
1937804
MDL number:
assay
≥98%
useful pH range
7.4-8.8
pKa (25 °C)
8.1
SMILES string
OCC(CO)(CO)NCC(O)=O
InChI
1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
InChI key
SEQKRHFRPICQDD-UHFFFAOYSA-N
General description
Tricine is one of the Good′s buffers that derives its name from tris and glycine. It has a pKa value of 7.8 and commonly used in animal tissue culture. It is a very useful buffer for the separation of small proteins in the range of 1 to 100 kDa.
Application
Tricine is a buffer useful in the physiological pH range 7.4-8.8 and pKa=8.15 at 20°C. It can also be used in many electrophoretic techniques for the analysis of proteins.
Buffer component for separation of low molecular weight peptides.
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Bioluminescent determination of 0.1 picomole amounts of guanine nucleotides
Ford S R, et al.
Journal of Bioluminescence and Chemiluminescence, 9(4), 251-265 (1994)
Tris-tricine and Tris-borate buffer systems provide better estimates of human mesothelial cell intermediate filament protein molecular weights than the standard Tris-glycine system
Patton W F, et al.
Analytical biochemistry, 197(1), 25-33 (1991)
Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa
Gels T, et al.
Analytical Biochemistry, 166(1), 368-379 (1987)