185906
Formamide
reagent grade, 98%
Synonym(s):
Amide C1, Formic amide
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About This Item
Linear Formula:
HCONH2
CAS Number:
Molecular Weight:
45.04
EC Number:
200-842-0
UNSPSC Code:
12352111
PubChem Substance ID:
Beilstein/REAXYS Number:
505995
MDL number:
Assay:
98%
Grade:
reagent grade
Bp:
210 °C (lit.)
Vapor pressure:
0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)
grade
reagent grade
vapor density
1.55 (vs air)
vapor pressure
0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)
assay
98%
form
liquid
autoignition temp.
932 °F
expl. lim.
2.7-19 % (lit.)
dilution
(for analytical testing)
refractive index
n20/D 1.447 (lit.)
bp
210 °C (lit.)
mp
2-3 °C (lit.)
density
1.134 g/mL at 25 °C (lit.)
SMILES string
NC=O
InChI
1S/CH3NO/c2-1-3/h1H,(H2,2,3)
InChI key
ZHNUHDYFZUAESO-UHFFFAOYSA-N
Application
Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.
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signalword
Danger
hcodes
Hazard Classifications
Carc. 2 - Repr. 1B - STOT RE 2 Oral
target_organs
Blood
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 1
flash_point_f
305.6 °F
flash_point_c
152 °C
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
Regulatory Information
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Solubilization in formamide protects RNA from degradation.
Chomczynski P.
Nucleic Acids Research, 20(14), 3791-3791 (1992)
Microwave spectrum and molecular structure of formamide.
Costain CC and Dowling JM.
J. Chem. Phys. , 32(1), 158-165 (1960)
J Casey et al.
Nucleic acids research, 4(5), 1539-1552 (1977-01-01)
The thermal stabilities of RNA:DNA hybrids are substantially greater than those of DNA:DNA duplexes in aqueous electrolyte solutions containing high concentrations of formamide. Association rates to form DNA:DNA duplexes and DNA:RNA hybrids have been measured in these solvents. There is