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Merck
CN

200182

Sigma-Aldrich

Nitrazine Yellow

indicator grade, Dye content 85 %

Synonym(s):

2-(2,4-Dinitrophenylazo)-1-hydroxynaphthalene-3,6-disulfonic acid disodium salt, 2-(2,4-Dinitrophenylazo)naphthol-3,6-disulfonic acid disodium salt, Nitrazol Yellow

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About This Item

Empirical Formula (Hill Notation):
C16H8N4Na2O11S2
CAS Number:
5423-07-4
Molecular Weight:
542.36
Colour Index Number:
14890
EC Number:
226-549-8
MDL number:
MFCD00003938
UNSPSC Code:
12171500
PubChem Substance ID:
24851961
NACRES:
NA.47

Key Documents

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grade

indicator grade

Assay

>90.0% (HPLC)

form

powder

composition

Dye content, 85%

solubility

H2O: 1 mg/mL

λmax

586 nm

ε (extinction coefficient)

≥35000 at 587-591 nm in 0.1 M NaOH at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2[N+]([O-])=O)[N+]([O-])=O)c(cc3cc(ccc13)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C16H10N4O11S2.2Na/c21-16-11-3-2-10(32(26,27)28)5-8(11)6-14(33(29,30)31)15(16)18-17-12-4-1-9(19(22)23)7-13(12)20(24)25;;/h1-7,21H,(H,26,27,28)(H,29,30,31);;/q;2*+1/p-2/b18-17+;;

InChI key

XBLIFEQTVVSTIM-QIKYXUGXSA-L

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Application

Nitrazine Yellow has been used in dye-protein binding assay.

Biochem/physiol Actions

Nitrazine Yellow ( 2-(2,4-dinitrophenylazo)-1-hydroxynaphthalene-3,6-disulfonic acid disodium salt) is a pH-sensitive azo dye. It changes color from yellow to blue in pH range 6.0–7.0 and is widely used in microscopy. Throat swab immersed in nitrazine yellow is used to identify pH variations in the vagina before and after amniotomy.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
MKCD1402
MKBQ6886V
MKBG8083V
MKBD9527V
36296KJ

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Gilbert G G Donders et al.
Infectious diseases in obstetrics and gynecology, 2010, 504972-504972 (2010-10-19)
Accessing vaginal pH is fundamental during gynaecological visit for the detection of abnormal vaginal flora (AVF), but use of pH strips may be time-consuming and difficult to interpret. The aim of this study was to evaluate the VS-SENSE test (Common
S L Bennett et al.
American journal of perinatology, 10(2), 101-104 (1993-03-01)
Most studies investigating the usefulness of ferning and nitrazine methods for detecting the presence of amniotic fluid following ruptured amniotic membranes have employed samples from vaginal pooling. Reported accuracies are 84 to 100% for the ferning test and 87 to
"Fig leaf" ferning and positive Nitrazine testing: semen as a cause of misdiagnosis of premature rupture of membranes.
J A McGregor et al.
American journal of obstetrics and gynecology, 151(8), 1142-1143 (1985-04-15)
The influence of a polyamide matrix on the halochromic behaviour of the pH-sensitive azo dye Nitrazine Yellow
Schueren L V D
Dyes and Pigments, 94, 443-451 (2012)
USE OF THE NITRAZINE YELLOW SWAB TEST IN THE DIAGNOSIS OF RUPTURED MEMBRANES
Mills AM
British Journal of Obstetrics and Gynaecology, 84, 134-180 (1977)
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