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Merck
CN

200220

Sigma-Aldrich

Azure II eosinate

powder

Synonym(s):

Azure II-eosin; Methylene blue, eosinate of Azure II

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About This Item

CAS Number:
53092-85-6
MDL number:
MFCD00084753
UNSPSC Code:
12171500
PubChem Substance ID:
24851351
NACRES:
NA.47

Key Documents

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form

powder

Quality Level

100

technique(s)

microbe id | staining: suitable

color

dark green

solubility

soluble, hazy to turbid

λmax

524 nm (2nd)
647 nm

Amax

≥0.50 at 645-655 nm in methanol at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[Cl-].[Cl-].CNc1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C.CN(C)c4ccc5N=C6C=C\C(C=C6Sc5c4)=[N+](/C)C.[O-]C(=O)c7ccccc7C8=C9C=C(Br)C(=O)C(Br)=C9Oc%10c(Br)c([O-])c(Br)cc8%10

InChI

1S/C20H8Br4O5.C16H18N3S.C15H15N3S.2ClH.2Na/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;1-16-10-4-6-12-14(8-10)19-15-9-11(18(2)3)5-7-13(15)17-12;;;;/h1-6,25H,(H,27,28);5-10H,1-4H3;4-9H,1-3H3;2*1H;;/q;+1;;;;2*+1/p-3

InChI key

CSDDIABEPMTRIP-UHFFFAOYSA-K

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General description

Azure II Eosinate is the eosinate of the dye Azure II which is a mixture of the dyes methylene blue and azure B in equal proportions. This compound is a key ingredient in Giemsa stain, which is widely utilized for staining blood elements and facilitating the visualization of cellular components under a microscope.

Application

Azure II Eosinate is used for human-medical cell staining and serves the purpose of the investigation of sample material of human origin (by fixing, embedding where necessary, staining, counterstaining, mounting) in hematological and clinico-cytological specimen materials, for example smears of whole blood and bone marrow.

Biochem/physiol Actions

The staining of the nuclei is due of the molecular interaction of the Eosin Y dye and a complex of Azure B with DNA. Both dyes assemble to an Eosin Y- Azure B-DNA complex and the intensity of the resulting stain depends on the content of Azure B and the ratio of Azure B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances.

Features and Benefits

Chief ingredient of Giemsa Stain.

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H318

Precautionary Statements

P280 - P305 + P351 + P338

Hazard Classifications

Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBS7660
SHBR5247
SHBR5246
SHBQ8743
SHBP1065

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The Giemsa stain: its history and applications
Juan Jose Barcia
International Journal of Surgical Pathology, 15(3), 292-296 (2007)

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