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216720

4′,5′-Dibromofluorescein

Dye content 95 %

Synonym(s):

Solvent Red 72

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About This Item

Empirical Formula (Hill Notation):
C20H10Br2O5
CAS Number:
596-03-2
Molecular Weight:
490.10
EC Number:
209-876-0
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24852976
MDL number:
MFCD00005042
Colour Index Number:
45370:1
Beilstein/REAXYS Number:
57189
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assay

>95% (TLC)

form

powder

composition

Dye content, 95%

mp

270-273 °C (lit.)

λmax

450 nm

ε (extinction coefficient)

≥5000 at 445-451 nm, ≥50000 at 227-233 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1ccc2c(Oc3c(Br)c(O)ccc3C24OC(=O)c5ccccc45)c1Br

InChI

1S/C20H10Br2O5/c21-15-13(23)7-5-11-17(15)26-18-12(6-8-14(24)16(18)22)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H

InChI key

ZDTNHRWWURISAA-UHFFFAOYSA-N

General description

4′,5′-Dibromofluorescein (DBF) is a fluorone dye. Fluorone dyes are hydroxyl-xanthene dyes (homologues of fluorescein) and have been the most widely used class of organic fluorophores for labeling and sensing biomolecules. DBF is routinely used as a ligand for studying the properties of protein using spectral analysis.

Application

4′,5′-Dibromofluorescein has been used to stain proteins in 1-D and 2-D sodium dodecyl sulfatepolyacrylamide gel electrophoresis SDS-PAGE. This method is rapid, economical, and highly sensitive in comparison with other proteomic research staining methods.

pictograms

Skull and crossbones
GHS06

signalword

Danger

hcodes

H301

Hazard Classifications

Acute Tox. 3 Oral

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges

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