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Merck
CN

216720

4′,5′-Dibromofluorescein

Dye content 95 %

Synonym(s):

Solvent Red 72

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About This Item

Empirical Formula (Hill Notation):
C20H10Br2O5
CAS Number:
596-03-2
Molecular Weight:
490.10
EC Number:
209-876-0
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24852976
MDL number:
MFCD00005042
Colour Index Number:
45370:1
Beilstein/REAXYS Number:
57189

Key Documents

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Product Name

4′,5′-Dibromofluorescein, Dye content 95 %

form

powder

InChI

1S/C20H10Br2O5/c21-15-13(23)7-5-11-17(15)26-18-12(6-8-14(24)16(18)22)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H

SMILES string

Oc1ccc2c(Oc3c(Br)c(O)ccc3C24OC(=O)c5ccccc45)c1Br

InChI key

ZDTNHRWWURISAA-UHFFFAOYSA-N

assay

>95% (TLC)

composition

Dye content, 95%

mp

270-273 °C (lit.)

λmax

450 nm

ε (extinction coefficient)

≥5000 at 445-451 nm
≥50000 at 227-233 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

100

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Application

4′,5′-Dibromofluorescein has been used to stain proteins in 1-D and 2-D sodium dodecyl sulfatepolyacrylamide gel electrophoresis SDS-PAGE. This method is rapid, economical, and highly sensitive in comparison with other proteomic research staining methods.

General description

4′,5′-Dibromofluorescein (DBF) is a fluorone dye. Fluorone dyes are hydroxyl-xanthene dyes (homologues of fluorescein) and have been the most widely used class of organic fluorophores for labeling and sensing biomolecules. DBF is routinely used as a ligand for studying the properties of protein using spectral analysis.

pictograms

Skull and crossbones
GHS06

signalword

Danger

hcodes

H301

Hazard Classifications

Acute Tox. 3 Oral

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges

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Certificates of Analysis (COA)

Lot/Batch Number
10812ENV
10812EN

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Gzona Bajraktari-Sylejmani et al.
European journal of drug metabolism and pharmacokinetics, 45(6), 809-815 (2020-07-15)
Citrus flavonoids are not only components of daily nutrition, they are also promoted as dietary supplements and are important ingredients in traditional medicines. Interactions of flavonoids with synthetic drugs represent an often neglected issue. We therefore investigated in vitro whether
Marc Le Vée et al.
Journal of biochemical and molecular toxicology, 33(10), e22379-e22379 (2019-08-01)
The interactions of six neonicotinoid pesticides and one neonicotinoid metabolite with drug transporters have been characterized in vitro. Acetamiprid, clothianidin, imidacloprid, nitenpyram, thiacloprid and its metabolite thiacloprid amide, and thiamethoxam, each used at 100 µM, did not impair activity of the

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