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Merck
CN

292648

Sigma-Aldrich

Dihydrofluorescein diacetate

97%

Synonym(s):

Diacetyldihydrofluorescein

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About This Item

Empirical Formula (Hill Notation):
C24H18O7
CAS Number:
35340-49-9
Molecular Weight:
418.40
Beilstein:
364167
MDL number:
MFCD00005056
UNSPSC Code:
12171500
PubChem Substance ID:
24857603
NACRES:
NA.47

Key Documents

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Assay

97%

mp

213-215 °C (lit.)

solubility

acetone: 50 mg/mL

fluorescence

λex 272 nm in methanol
λex 490; λem 514 (Reaction product)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CC(=O)Oc1ccc2C(c3ccc(OC(C)=O)cc3Oc2c1)c4ccccc4C(O)=O

InChI

1S/C24H18O7/c1-13(25)29-15-7-9-19-21(11-15)31-22-12-16(30-14(2)26)8-10-20(22)23(19)17-5-3-4-6-18(17)24(27)28/h3-12,23H,1-2H3,(H,27,28)

InChI key

YKSJJXGQHSESKB-UHFFFAOYSA-N

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General description

Dihydrofluorescein diacetate (H2FDA) is an esterified derivative of dihydrofluorescein. H2FDA is one of the widely used fluorescent probes employed in the intracellular reactive oxygen species (ROS) detection.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
MKBF9112V
MKBF9112
13519BH
11402TF
08008KQ

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Floriane L'Haridon et al.
PLoS pathogens, 7(7), e1002148-e1002148 (2011-08-11)
Wounded leaves of Arabidopsis thaliana show transient immunity to Botrytis cinerea, the causal agent of grey mould. Using a fluorescent probe, histological staining and a luminol assay, we now show that reactive oxygen species (ROS), including H(2)O(2) and O(2) (-)
Matthieu Dallons et al.
Frontiers in pharmacology, 11, 79-79 (2020-03-11)
Doxorubicin (DOX) is an anticancer drug widely used in oncology. The main limitation to DOX treatments though is due to the cumulative dose that may lead to cardiotoxicity. Clinically, DOX-induced cardiomyopathy develops as a progressive heart failure consecutive to a
Abhishek K Verma et al.
Frontiers in microbiology, 9, 1489-1489 (2018-07-24)
Chandipura Virus (CHPV) a negative-stranded RNA virus belonging to the Rhabdoviridae family, has been previously reported to bring about neuronal apoptosis by stimulating oxidative stress. Our in silico data suggested the involvement of Angiotensin II in intracellular Ca2+ secretion within
Chandan K. Sen, Lester Packer
Redox Cell Biology and Genetics, (2002)
Ekrem Sulukan et al.
Chemosphere, 180, 77-85 (2017-04-10)
In this study, it has been investigated that the effects of glyphosate, which is a herbicide within organophosphate and unselective widely used in agriculture on enzyme activity of carbonic anhydrase, production of reactive oxygen species, cell apoptosis and body morphology
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