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Merck
CN

323829

Methyl Green

zinc chloride salt, <0.5% crystal violet, Dye content 80 %

Synonym(s):

Ethyl Green

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About This Item

Linear Formula:
C27H35BrClN3 · ZnCl2
CAS Number:
7114-03-6
Molecular Weight:
653.24
EC Number:
230-415-4
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24859418
MDL number:
MFCD00151094
Colour Index Number:
42590
Beilstein/REAXYS Number:
3914106

Key Documents

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Product Name

Methyl Green, zinc chloride salt, <0.5% crystal violet, Dye content 80 %

InChI key

RRKSQWOQZFIQKW-UHFFFAOYSA-J

InChI

1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4

SMILES string

[Cl-].[Br-].Cl[Zn]Cl.CC[N+](C)(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](\C)C

assay

>75.0% (HPLC)

form

solid

composition

Dye content, 80%

technique(s)

titration: suitable

impurities

<0.5% crystal violet

pH

0.2-1.8, yellow to blue

mp

>300 °C (lit.)

solubility

water: 1 mg/mL

λmax

420 nm (2nd)
635 nm

ε (extinction coefficient)

≥14000 at 311-317 in water at 0.005 g/L
≥50000 at 629-635 in water at 0.005 g/L
≥9000 at 420-426 in water at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Related Categories

Application

Used in the Methyl Green-Pyronin methods to demonstrate DNA and RNA in tissue sections.

General description

Methyl Green is a triphenylmethane dye which is mainly used in combination with pyronin. It is suitable for the detection of DNA and RNA.

Other Notes

Zinc chloride salt

signalword

Danger

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1B - STOT SE 3

target_organs

Respiratory system

Storage Class

8A - Combustible corrosive hazardous materials

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBM8610
MKCM9394
MKCN1926
MKCJ7721
MKCJ4715

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