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Merck
CN

642983

Sigma-Aldrich

4-Nitrophenylhydrazine

96%

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About This Item

Linear Formula:
O2NC6H4NHNH2
CAS Number:
Molecular Weight:
153.14
Beilstein:
608107
EC Number:
MDL number:
UNSPSC Code:
12352100
PubChem Substance ID:
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Assay

96%

form

solid

contains

≥30% water as stabilizer

mp

156 °C (dec.) (lit.)

SMILES string

NNc1ccc(cc1)[N+]([O-])=O

InChI

1S/C6H7N3O2/c7-8-5-1-3-6(4-2-5)9(10)11/h1-4,8H,7H2

InChI key

KMVPXBDOWDXXEN-UHFFFAOYSA-N

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Pictograms

Exploding BombExclamation mark

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Expl. 1.1 - Eye Irrit. 2 - Flam. Sol. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

1 - Explosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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V Steinebach et al.
Analytical biochemistry, 230(1), 159-166 (1995-09-01)
Pig kidney diamine oxidase was purified to homogeneity. The reaction product of the cofactor with p-nitrophenylhydrazine (pNPH) was liberated with pronase treatment and purified. 1H NMR, uv/vis, and electrospray tandem mass spectroscopy revealed it to be a dipeptide with the
Analytical and mechanistic aspects of the electrochemical oxidation of keto steroids derivatized with phenylhydrazine, (4-nitrophenyl)hydrazine, and (2,4-dinitrophenyl)hydrazine.
A M Bond et al.
Analytical chemistry, 60(10), 1023-1027 (1988-05-15)
I Frébort et al.
European journal of biochemistry, 225(3), 959-965 (1994-11-01)
Interactions of two distinct quinoprotein amine oxidases from Aspergillus niger, AO-I and AO-II, with active-site covalent modifiers have been investigated. Both enzymes are inhibited similarly by phenylhydrazine or p-nitrophenylhydrazine, forming an orange Schiff base with a carbonyl group of topaquinone
N Nakamura et al.
The Journal of biological chemistry, 271(9), 4718-4724 (1996-03-01)
Resonance Raman spectroscopy is an excellent technique for providing structural information on the 2,4, 5-trihydroxyphenylalanine quinone (TPQ) cofactor in copper-containing amine oxidases. This technique has been used to investigate the copper- and O2-dependent biosynthesis of the TPQ cofactor in phenylethylamine
T Huque et al.
Comparative biochemistry and physiology. B, Comparative biochemistry, 86(1), 135-139 (1987-01-01)
The total plasmalogen content of lingual and other tissues was analyzed using the iodine-addition (Method 1), the p-nitrophenylhydrazone (Method 2), and the two-dimensional thin layer chromatography procedure (Method 3). Methods 1 and 2 were simple, rapid and reproducible, yielding values

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