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Merck
CN

78440

o-Phenylenediamine dihydrochloride

≥99.0% (AT)

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About This Item

Linear Formula:
C6H4(NH2)2·2HCl
CAS Number:
Molecular Weight:
181.06
EC Number:
210-418-7
UNSPSC Code:
12352100
PubChem Substance ID:
Beilstein/REAXYS Number:
3912045
MDL number:
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assay

≥99.0% (AT)

mp

258 °C (dec.) (lit.)

SMILES string

Cl[H].Cl[H].Nc1ccccc1N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

InChI key

RIIWUGSYXOBDMC-UHFFFAOYSA-N

General description

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Disclaimer

may discolor to violet on storage


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Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges

wgk

WGK 3

Regulatory Information

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Fatemeh Adiliaghdam et al.
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While interferon (IFN) responses are critical for mammalian antiviral defense, induction of antiviral RNA interference (RNAi) is evident. To date, individual functions of the mammalian RNAi and micro RNA (miRNA) effector proteins Argonautes 1-4 (AGO1-AGO4) during virus infection remain undetermined.
Luisa Benerini Gatta et al.
Cancers, 11(9) (2019-08-24)
Urothelial bladder cancer (UBC) are classified into luminal and basal subtypes showing distinct molecular features and clinical behaviour. Recent in silico data have proposed the activation on the Signal Transducer and Activator of Transcription 3 (STAT3) as relevant transcription factor
Ayuko Hoshino et al.
Cell, 182(4), 1044-1061 (2020-08-17)
There is an unmet clinical need for improved tissue and liquid biopsy tools for cancer detection. We investigated the proteomic profile of extracellular vesicles and particles (EVPs) in 426 human samples from tissue explants (TEs), plasma, and other bodily fluids.



Global Trade Item Number

SKUGTIN
649317-1G04061832731476
674656-5ML04061838128768
674656-25ML04061838128751
457698-25ML04061832342375
457698-5ML04061838127907
649317-10G04061832731469