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About This Item
Linear Formula:
C6H4(NH2)2·2HCl
CAS Number:
Molecular Weight:
181.06
EC Number:
210-418-7
UNSPSC Code:
12352100
PubChem Substance ID:
Beilstein/REAXYS Number:
3912045
MDL number:
assay
≥99.0% (AT)
mp
258 °C (dec.) (lit.)
SMILES string
Cl[H].Cl[H].Nc1ccccc1N
InChI
1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H
InChI key
RIIWUGSYXOBDMC-UHFFFAOYSA-N
General description
o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.
Disclaimer
may discolor to violet on storage
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signalword
Danger
Hazard Classifications
Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges
wgk
WGK 3
Regulatory Information
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 649317-1G | 04061832731476 |
| 674656-5ML | 04061838128768 |
| 674656-25ML | 04061838128751 |
| 457698-25ML | 04061832342375 |
| 457698-5ML | 04061838127907 |
| 649317-10G | 04061832731469 |


