858811
5-Bromo-2′-deoxyuridine
97%
Synonym(s):
5-BrdU, 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil, 5-Bromouracil deoxyriboside, BUdR
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About This Item
Assay
97%
optical activity
[α]22/D +23.0°, c = 1 in H2O
mp
191-194 °C (dec.) (lit.)
storage temp.
−20°C
SMILES string
OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O
InChI
1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1
InChI key
WOVKYSAHUYNSMH-RRKCRQDMSA-N
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Biochem/physiol Actions
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Muta. 1B - Repr. 2
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 2
Personal Protective Equipment
Regulatory Information
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
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What is the stability of Product 858811, 5-Bromo-2´-deoxyuridine (BrdU)?
The comparable Sigma product, B5002, has a recommended retest specification of 2 years. We have observed by TLC that the material is unchanged after 3 years. Solutions at neutral pH should be stable if stored frozen for up to 6 months.
What can I use to detect BrdU, 5-Bromo-2´-deoxyuridine, in vivo and in vitro?
For the immunohistochemical detection of BrdU, we recommend the monoclonal anti-BrdU antibody, Product No. B2531.
What are the hazards associated with Product 858811, 5-Bromo-2´-deoxyuridine (BrdU)?
BrdU is a known carcinogen, and caution should be exercised when handling. Please refer to the MSDS.
How long do I treat cells with Product 858811, 5-Bromo-2´-deoxyuridine (BrdU) to incorporate it into the DNA?
Actively growing cells may be treated with 10 μM BrdU for 1 hour.
How does Product 858811, 5-Bromo-2´-deoxyuridine (BrdU), work as a thymidine analog?
BrdU replaces thymidine during the S-phase of cell division.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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