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860891

Sigma-Aldrich

Bromocresol Purple sodium salt

indicator grade, Dye content 90 %

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Synonym(s):
5′,5″-Dibromo-o-cresolsulfonphthalein sodium salt
Empirical Formula (Hill Notation):
C21H15Br2NaO5S
CAS Number:
62625-30-3
Molecular Weight:
562.20
EC Number:
263-655-3
MDL number:
MFCD00148896
PubChem Substance ID:
24888522
NACRES:
NA.47

grade

indicator grade

Quality Level

200

form

powder

composition

Dye content, 90%

technique(s)

titration: suitable

color

brown to very dark brown, to Very Dark Red

visual transition interval

5.2-6.8, yellow to purple

mp

255 °C (dec.) (lit.)

λmax

379 nm
585 nm (2nd)

ε (extinction coefficient)

≥47000 at 587-591 nm in 0.1 M NaOH
≥7000 at 375-381 nm in 0.1 M NaOH

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].Cc1cc(cc(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C)c([O-])c(Br)c4

InChI

1S/C21H16Br2O5S.Na/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21;/h3-10,24-25H,1-2H3;/q;+1/p-1

InChI key

WNOIORUCCYNQPE-UHFFFAOYSA-M

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General description

Bromocresol Purple, also known as dibromo-o-cresolsulfonphthalein, is an acidic dye and belongs to the phthalein and sulphonphthalein dyes group. The dye color changes from yellow to violet at pH 5.2-6.8. The monosodium salt of the dye is 8% soluble in water and 2% in ethanol. Bromocresol Purple, under alkaline conditions, forms a hydrophilic, moderately sized dianion that is purple.

Application

Bromocresol Purple is widely used as a vital stain to trace fluid diffusion in the lymphatic system. In addition, the dye is suitable to differentiate dead or live yeast cells. As a pH indicator, Bromocresol Purple is used in microbial culture media, both broth, and agar. In analytical experiments, e.g., to stain carbonic anhydrase isoenzyme on cellulose acetate electrophoresis membranes, bromocresol purple is used.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315 - H319 - H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Sabins RW
Handbook of Acid-Base Indicators (2007)
Roland Krivanek et al.
Biophysical journal, 94(5), 1890-1903 (2007-11-13)
The energetics of the individual reaction steps in the catalytic cycle of photosynthetic water oxidation at the Mn(4)Ca complex of photosystem II (PSII) are of prime interest. We studied the electron transfer reactions in oxygen-evolving PSII membrane particles from spinach
Senthilkumar Padmanaban et al.
Plant physiology, 144(1), 82-93 (2007-03-06)
Guard cell movement is induced by environmental and hormonal signals that cause changes in turgor through changes in uptake or release of solutes and water. Several transporters mediating these fluxes at the plasma membrane have been characterized; however, less is

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