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Merck
CN

861030

Auramine O

Dye content 85 %, certified by the BSC

Synonym(s):

4,4′-(Imidocarbonyl)bis(N,N-dimethylaniline) monohydrochloride, Basic Yellow 2, Pyoctaninum aureum

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About This Item

Empirical Formula (Hill Notation):
C17H21N3 · HCl
CAS Number:
2465-27-2
Molecular Weight:
303.83
EC Number:
219-567-2
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24888527
MDL number:
MFCD00012484
Colour Index Number:
41000
Beilstein/REAXYS Number:
4030061

Key Documents

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Product Name

Auramine O, Dye content 85 %, certified by the BSC

InChI key

KSCQDDRPFHTIRL-UHFFFAOYSA-N

InChI

1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H

SMILES string

Cl[H].CN(C)c1ccc(cc1)C(=N)c2ccc(cc2)N(C)C

agency

certified by the BSC

form

powder or chunks

composition

Dye content, 85%

technique(s)

microbe id | staining: suitable

mp

>250 °C (dec.) (lit.)

solubility

95% ethanol: 1 mg/mL, clear to hazy, yellow to very deep yellow

λmax

370 nm
434 nm (2nd)

ε (extinction coefficient)

≥12000 at 249-255 nm in water at 0.005 g/L
≥15000 at 367-373 nm in water at 0.005 g/L
≥36000 at 429-435 nm in water at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Related Categories

Application

Auramine O has been used to study the cuticle/cutin in the epidermis of the pollen presenter in Vangueria infausta.
Certified for use by fluorescence microscopy in Churukian′s modification of Truant′s fluorescent method for acid fast bacilli on paraffin sections.

Biochem/physiol Actions

Auramine O is used for the staining of acid-fast organisms, coccidia. Auramine O along with carbol generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis. Auramine O binds to the mycolic acid in the bacterial cell wall.

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
SHBR1437
SHBQ0337
SHBM7243
MKCN0419
MKCK2369

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Koneman's Color Atlas and Textbook of Diagnostic Microbiology (2006)
The Sigma-Aldrich Handbook of Stains, Dyes and Indicators,, 103-103 (1990)
Garcia LS
Practical Guide to Diagnostic Parasitology (2009)
Nadav Amdursky et al.
The journal of physical chemistry. B, 116(45), 13389-13395 (2012-10-24)
There is an indispensable need for a fluorescence marker for the detection of amyloid fibrils, where, at present, the most used marker is thioflavin-T (ThT). Here, we present the use of auramine-O (AuO) as a possible alternative to ThT. As
Sumita Dixit et al.
Journal of AOAC International, 94(6), 1874-1881 (2012-02-11)
The present method utilizes a simple pretreatment step, cleanup on polyamide SPE cartridges, and HPLC resolution on reversed-phase C18 for the detection of the three basic nonpermitted dyes encountered in food matrixes. Polyamide cartridges were chosen because both acidic and
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