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Merck
CN

861030

Auramine O

Dye content 85 %, certified by the BSC

Synonym(s):

4,4′-(Imidocarbonyl)bis(N,N-dimethylaniline) monohydrochloride, Basic Yellow 2, Pyoctaninum aureum

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About This Item

Empirical Formula (Hill Notation):
C17H21N3 · HCl
CAS Number:
2465-27-2
Molecular Weight:
303.83
EC Number:
219-567-2
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24888527
MDL number:
MFCD00012484
Colour Index Number:
41000
Beilstein/REAXYS Number:
4030061

Key Documents

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InChI key

KSCQDDRPFHTIRL-UHFFFAOYSA-N

InChI

1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H

SMILES string

Cl[H].CN(C)c1ccc(cc1)C(=N)c2ccc(cc2)N(C)C

agency

certified by the BSC

form

powder or chunks

composition

Dye content, 85%

technique(s)

microbe id | staining: suitable

mp

>250 °C (dec.) (lit.)

solubility

95% ethanol: 1 mg/mL, clear to hazy, yellow to very deep yellow

λmax

370 nm, 434 nm (2nd)

ε (extinction coefficient)

≥12000 at 249-255 nm in water at 0.005 g/L, ≥15000 at 367-373 nm in water at 0.005 g/L, ≥36000 at 429-435 nm in water at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Related Categories

Application

Auramine O has been used to study the cuticle/cutin in the epidermis of the pollen presenter in Vangueria infausta.
Certified for use by fluorescence microscopy in Churukian′s modification of Truant′s fluorescent method for acid fast bacilli on paraffin sections.

Biochem/physiol Actions

Auramine O is used for the staining of acid-fast organisms, coccidia. Auramine O along with carbol generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis. Auramine O binds to the mycolic acid in the bacterial cell wall.

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
SHBR1437
SHBQ0337
SHBM7243
MKCN0419
MKCK2369

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Garcia LS
Practical Guide to Diagnostic Parasitology (2009)
Koneman's Color Atlas and Textbook of Diagnostic Microbiology (2006)
The Sigma-Aldrich Handbook of Stains, Dyes and Indicators,, 103-103 (1990)
The epidermal cell structure of the secondary pollen presenter in Vangueria infausta (Rubiaceae: Vanguerieae) suggests a functional association with protruding onci in pollen grains.
PLoS ONE, 9, e96405-e96405 (2014)
M Habtamu et al.
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease, 16(3), 402-407 (2012-05-30)
Mawenzi Regional Hospital, northern Tanzania. To determine the value of light-emitting diode (LED) microscopy in diagnosing tuberculosis (TB) on bleach-treated and direct sputum smears. Sputum samples were collected from patients suspected of pulmonary TB who presented consecutively at the laboratory
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