861049
Azure A chloride
certified by the BSC, Dye content 70 %
Synonym(s):
Methylene blue
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About This Item
Empirical Formula (Hill Notation):
C14H14ClN3S
CAS Number:
Molecular Weight:
291.80
EC Number:
208-510-7
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
MDL number:
Colour Index Number:
52005
Beilstein/REAXYS Number:
3922287
agency
certified by the BSC
Quality Level
form
powder
composition
Dye content, 70%
color
blue to very dark blue, very dark green
mp
290 °C (dec.) (lit.)
solubility
H2O: 1 mg/mL
λmax
633 nm
ε (extinction coefficient)
≥11000 at 242-246 nm in H2O at -1, ≥26000 at 287-291 nm in H2O, ≥34000 at 620-634 μm in H2O
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
NC1=CC(SC(C2=N3)=CC(C=C2)=[N+](C)C)=C3C=C1.[Cl-]
InChI
1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H
InChI key
NALREUIWICQLPS-UHFFFAOYSA-N
Application
Azure A has been used to determine the amount of charged dextran sulfate in the nanoparticles for the study involving incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles.
Certified for use in Lillie′s modified Nocht′s method and the NCCLS method for blood smears.
Biochem/physiol Actions
The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. Azure A is a main component of the azure B/polychromed methylene blue-eosin stains family, also referred to as the Romanowsky stain. Azure A is a basic cationic dye.
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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How Romanowsky stains work and why they remain valuable - including a proposed universal Romanowsky staining mechanism and a rational troubleshooting scheme.
Horobin RW
Biotechnic & Histochemistry, 86, 36-51 (2011)
Quantification and characterization of glycosaminoglycans at the nanogram level by a combined azure A-silver staining in agarose gels.
van de Lest CH, et al.
Analytical Biochemistry, 221, 356-361 (1994)
Paula Zaman et al.
International journal of nanomedicine, 11, 6149-6159 (2016-12-07)
Incorporation of proteins into dextran sulfate (DS)-chitosan (CS) nanoparticles (DSCS NPs) is commonly performed using entrapment procedures, in which protein molecules are mixed with DS and CS until particle formation occurs. As DS is an analog of heparin, the authors
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 861049-25G | 04061833063361 |
| 861049-5G | 04061833040102 |